FastQCFastQC Report
Fri 14 Jul 2023
SRR9691476_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691476_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3584209
Total Bases272.3 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCAGCCTGATGCGTGGACTGAGGACAACGGAGATCGGAAGAGCACAC973272.7154387481310382No Hit
AGTAGTCCAGCCTGATGCGTGGACTGAGGACAACGGAGATCGGAAGAGCA780962.1788907957097368No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG576991.6098112582162478No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA342620.9559152382017901No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA328630.91688291614691Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA294090.8205157679142037No Hit
ATTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTACAGATCGGAA183890.5130560187756908No Hit
ATTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTACAAATATGCC182060.5079502897291982No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA167070.4661279518019178No Hit
ATGGGTCGTATCGTATTTTTGGGTAACACAATCCATCAAAGAACGAGATC158090.4410736092677631No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA154870.43208975815863415No Hit
TTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTACAAATATGCCA151900.4238034110176053No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCAC143300.3998092745149627No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG138070.38521749150230916No Hit
AATGGGTCGTATCGTATTTTTGGGTAACACAATCCATCAAAGAACGAGAT132740.37034670690241556No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG129800.3621440602375587No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACTCATCTCGTATG127040.3544436164297339TruSeq Adapter, Index 23 (97% over 49bp)
ATTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTACAAGATCGGA126680.35343921071566975No Hit
ATTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCAGATCGGAAGAGCA120780.336978117068508No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC110450.3081572531066129No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA109350.3050882356469726No Hit
AAATTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTACAAATATG106880.29819689644214387No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG104430.29136135755476317No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC94580.2638797012116202Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA93040.25958307676812375No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG88980.24825561232617852No Hit
TTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCAGATCGGAAGAGCAC88210.2461073001044303No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG87210.24331728423202997No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC83010.2315992175679487No Hit
ATTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTAGATCGGAAGAG82180.2292835043938565No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA81870.2284185994734124Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA81020.22604708598187215No Hit
AGTAGTCCAGCCTGATGCGTGGACTGAGGACAACGGTAGATCGGAAGAGC77510.21625413026974707No Hit
AGTTGTTAAAGAATTATCGTCATTTACATTAGGCACAGGTGAGATCGGAA76300.21287821106414276No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG74920.20902798916023033No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC74900.20897218884278232No Hit
AATTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTACAGATCGGA74680.20835838535085424No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA74380.20752138058913416Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTACAGATCGGAAG72590.20252725217753761No Hit
AAATTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTACAGATCGG71500.1994861348766213No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA71130.1984538290038332Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA71070.19828642805148916No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA67320.18782386852998806No Hit
TTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTACAAATATGCCAAC66200.18469905075289972No Hit
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA62840.17532459742163475No Hit
AATTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTACAAATATGC62480.17432019170757063No Hit
AGTCCAGCCTGATGCGTGGACTGAGGACAACGGTTAGATCGGAAGAGCAC61990.17295308393009448No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG61950.17284148329519847No Hit
TTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTACAAATATGCCAA61210.17077687154962226No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC60950.17005146742279817No Hit
AAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCG60660.1692423628198021No Hit
AGTTGTTAAAGAATTATCGTCATTTACATTAGGCACAGGAGATCGGAAGA58510.16324382869414145No Hit
AGTCCAGCCTGATGCGTGGACTGAGGACAACGGTAGATCGGAAGAGCACA57970.16173722012304528No Hit
ATTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTAGATCGGAAGA56430.15744059567954882No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCA56090.15649199028293273No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG54100.15093985869685614Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCACA53220.14848464472914386No Hit
ATTATCGTCATTTACATTAGGCACAGGTGTACAAGATCGGAAGAGCACAC52730.1471175369516677No Hit
ATTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTACAAAAGATCG52430.14628053218994763No Hit
ATTTTTCGTGGGCTTGAGATTTGTTGTTAGATCGGAAGAGCACACGTCTG51870.14471812330140346Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACAC51370.1433231153652033No Hit
AGTCCAGCCTGATGCGTGGACTGAGGACAACGAGATCGGAAGAGCACACG49750.13880328965191485No Hit
TTTCGTGGGCTTGAGATTTGTTGTTATGGACCCAGATCGGAAGAGCACAC49030.13679447822378663No Hit
AGTTGTTAAAGAATTATCGTCATTTACATTAGGCACAGGTGTACAAGATC48170.13439506457352235No Hit
AATTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTACAAGATCGG46410.12948463663809784No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC46170.12881503282872175No Hit
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG45880.12800592822572568No Hit
AAGAATTATCGTCATTTACATTAGGCACAGGTGTACAAGATCGGAAGAGC45470.12686202171804156No Hit
AAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACA43420.12114248917962094No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGA43170.12044498521152087No Hit
AGTAGTCCAGCCTGATGCGTGGACTGAGGACAACGAGATCGGAAGAGCAC42420.11835247330722065No Hit
TTTCGTGGGCTTGAGATTTGTTGTTAGATCGGAAGAGCACACGTCTGAAC42280.1179618710850846Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC42080.11740386791060453No Hit
TAGTCCAGCCTGATGCGTGGACTGAGGACAACGGAGATCGGAAGAGCACA41680.11628786156164443No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAA40860.11400004854627617No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA40830.11391634807010417Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAATTATCGTCATTTACATTAGGCACAGGTGTACAAGATCGGAAGAGCA39930.1114053337849439No Hit
TTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCAGATCGGAAGAGCACA39510.11023352711853576No Hit
AGGACGGGGATCAAGAGAGGTCAGATCGGAAGAGCACACGTCTGAACTCC39500.11020562695981179Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATGCGTGGACTGAGGACAACGGAGATCGGAAGAGCACACGTCTGAACT38120.10635540505589935Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG37590.10487669664352721No Hit
AATTATCGTCATTTACATTAGGCACAGGTGTACAAGATCGGAAGAGCACA37440.10445819426266716No Hit
ATTTTTCGTGGGCTTGAGATTTGTTGTTAAGATCGGAAGAGCACACGTCT36850.10281208489795099Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAATTATCGTCATTTACATTAGGCACAGGTGAGATCGGAAGAGCACAC36300.10127757616813081No Hit
TTTTTCGTGGGCTTGAGATTTGTTGTTAGATCGGAAGAGCACACGTCTGA35850.10002206902555069Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)