FastQCFastQC Report
Fri 14 Jul 2023
SRR9691481_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691481_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4365337
Total Bases331.7 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCAGCCTGATGCGTGGACTGAGGACAACGGAGATCGGAAGAGCACAC3934509.013049851592214No Hit
AGTAGTCCAGCCTGATGCGTGGACTGAGGACAACGGAGATCGGAAGAGCA2275235.212037466981358No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA708231.6223947887643038No Hit
ATTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCAGATCGGAAGAGCA591021.3538931816718847No Hit
TTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCAGATCGGAAGAGCAC439071.0058100898052087No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA333240.7633774895271545Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTCCAGCCTGATGCGTGGACTGAGGACAACGGAGATCGGAAGAGCACA298990.6849184839566796No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA296360.6788937486384212Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG279630.6405691015378652No Hit
TTTCGTGGGCTTGAGATTTGTTGTTAGATCGGAAGAGCACACGTCTGAAC258760.5927606505522941Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC257840.5906531385778463No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA235760.5400728511910993No Hit
AGTCCAGCCTGATGCGTGGACTGAGGACAACGAGATCGGAAGAGCACACG225010.515447031924454No Hit
TTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTACAAATATGCCA217330.4978538884855855No Hit
TTTCGTGGGCTTGAGATTTGTTGTTATGGACCCAGATCGGAAGAGCACAC212010.4856669714159525No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC200400.4590710866079755No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA187380.4292452106217687No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA177460.40652073367989683Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTACAAATATGCC172930.39614352797962676No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG168810.3867055395723171No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA166400.381184774508818No Hit
TTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCAGATCGGAAGAGCACA159500.36537843470045955No Hit
TTTTTCGTGGGCTTGAGATTTGTTGTTAGATCGGAAGAGCACACGTCTGA146390.33534638906457853Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTAGTCCAGCCTGATGCGTGGACTGAGGACAACGAGATCGGAAGAGCAC139870.3204105433326224No Hit
ATTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTACAGATCGGAA125110.286598720786047No Hit
ATTTTTCGTGGGCTTGAGATTTGTTGTTAGATCGGAAGAGCACACGTCTG114560.26243105629645547Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC113450.2598882972838065Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG111180.2546882405642451No Hit
TTTCGTGGGCTTGAGATTTGTTGTAGATCGGAAGAGCACACGTCTGAACT106380.24369252591495225Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCGTGGGCTTGAGATTTGTTGAGATCGGAAGAGCACACGTCTGAACTC105910.2426158621888757Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTCCAGCCTGATGCGTGGACTGAGGACAAGATCGGAAGAGCACACGTCT102120.2339338291637049Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTAGATCGGAAGAGC101850.23331532021468215No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA97660.22371697763540363Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTCGTGGGCTTGAGATTTGTTGTTAGATCGGAAGAGCACACGTCTGAA96830.2218156353106301Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC96610.22131166505587083Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTAGTCCAGCCTGATGCGTGGACTGAGGACAAGATCGGAAGAGCACACG94060.215470191648434No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG88780.2033749055342119No Hit
TTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTACAGATCGGAAG85200.19517393502494768No Hit
AGCCTGATGCGTGGACTGAGGACAACGGAGATCGGAAGAGCACACGTCTG81810.1874082115538846Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCAGATCGGAAGAGC80980.18550686922911105No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG79290.18163546136300587No Hit
CTGATGCGTGGACTGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT77700.17799313088542765Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC76110.17435080040784937Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTTTCGTGGGCTTGAGATTTGTTGAGATCGGAAGAGCACACGTCTGAAC76020.17414463075817516Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA68960.15797176712817362Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA68200.1562307789753689Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTAGTCCAGCCTGATGCGTGGACTGAGGACAACGGAGATCGGAAGAGC67070.15364220448501456No Hit
AGTTGTTAAAGAATTATCGTCATTTACATTAGGCACAGGAGATCGGAAGA66580.1525197252812326No Hit
TTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTAGATCGGAAGAGCA66540.1524280943258218No Hit
AAATTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTACAAATATG65530.15011441270169978No Hit
ATTTTTCGTGGGCTTGAGATTTGTTGTTAAGATCGGAAGAGCACACGTCT65360.149724981141204Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTACAGATCGGAAGAG64680.14816725489922083No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA62670.14356279938982947No Hit
ATTTTTCGTGGGCTTGAGATTTGTTGAGATCGGAAGAGCACACGTCTGAA62250.14260067435801635Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGCCTGATGCGTGGACTGAGGACAACGGAGATCGGAAGAGCACACGTC61930.14186762671473013No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT61300.14042443916701047No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA59490.13627813843467299No Hit
TTTTTCGTGGGCTTGAGATTTGTTGTAGATCGGAAGAGCACACGTCTGAA59200.13561381400794487Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTATCGTCATTTACATTAGGCACAGGTGAGATCGGAAGAGCACACGTCT58710.1344913348041629Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA57040.1306657424157631No Hit
TGATGCGTGGACTGAGGACAACGGAGATCGGAAGAGCACACGTCTGAACT55660.1275044744540914Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT54780.1254885934350544No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA54230.12422866779815624No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT53060.12154846235239113No Hit
AGTCCAGCCTGATGCGTGGACTGAGGACAACGGTTAGATCGGAAGAGCAC52630.12056342958172529No Hit
TTCGTGGGCTTGAGATTTGTTGTTAGATCGGAAGAGCACACGTCTGAACT52540.12035725993205107Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTAGATCGGAAGAG52120.11939513490023795No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC51390.11772286996399133Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTACAAATATGC50190.11497394130166813No Hit
ATTTTTCGTGGGCTTGAGATTTGTTGTTATGGACCCTTTACAAAAGATCG48960.11215628942278684No Hit
AGTCCAGCCTGATGCGTGGACTGAGGACAACGAAGATCGGAAGAGCACAC48780.11174395012343835No Hit
TGATGCGTGGACTGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC47210.10814743512356549Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG46240.10592538445485422No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACCATCTCGTATG46160.10574212254403267TruSeq Adapter, Index 6 (97% over 37bp)
ATTTTTCGTGGGCTTGAGATTTGTTGTAGATCGGAAGAGCACACGTCTGA45930.10521524455042074Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCAGCCTGATGCGTGGACTGAGGACAACAGATCGGAAGAGCACACGT45870.10507779811730457No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG45550.10434475047401838No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG45300.10377205700270103No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA44860.10276411649318255No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC44160.101160574773494No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)