FastQCFastQC Report
Fri 14 Jul 2023
SRR9691483_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691483_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2617764
Total Bases198.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGC542902.073907349936816No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA348101.3297608187751073No Hit
TGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGT340861.3021036273705346No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA294671.125655330274234No Hit
TGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACAC283091.0814191042431631No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC235890.9011125525448437No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA202580.7738665517594405Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG201370.7692442863451404No Hit
TTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCAC200020.7640872133622435No Hit
TGGTTGTGCAGCCAGATGCTTGGACAGAGGACAAGATCGGAAGAGCACAC184600.7051819797353772No Hit
TTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACA163110.6230890179557821No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG156600.5982204660160351No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA137280.5244170215496889No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC119230.45546504574132735Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGA110940.4237967975722792No Hit
TGGTTGTGCAGCCAGATGCTTGGACAGAGGACAATGGCAGATCGGAAGAG103720.39621600724893463No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA100290.3831132218183152Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC96890.3701250380095379Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC84380.32233616170136037No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC84210.32168675251092155Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG81500.3113344060045138No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC81500.3113344060045138Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA75570.2886814854203817Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGTTGTGCAGCCAGATGCTTGGACAGAGGACAATGGCTAGATCGGAAGA71550.27332486809353324No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA63070.24093080965281818Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGTCT61700.23569733558869324Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTTGTGTGTGAACGGACACTGAAATATTTTCAGATCGGAAGAGCACAC60600.23149527612114765No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG53800.2055189085035931No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG52070.19891021497736236No Hit
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG49540.18924547820200752No Hit
AGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAGCACACGTCTG49420.1887870717146389Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA48650.185845630087357Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA46080.17602809114954596No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT45930.1754550830403352No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTCACATCTCGTATG45630.17430906682191366TruSeq Adapter, Index 27 (97% over 37bp)
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA44390.16957219978577137No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC42100.16082427598515373Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT41730.15941085598243387No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA40650.15528519759611636No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG37850.14458904622418217No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA37820.14447444460234No Hit
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA35600.13599392458602072No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT34980.13362549106794958Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG33120.12652019051373614No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA32070.12250913374926083No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA30280.11567123697934574Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA29320.11200398508039684No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA28640.1094063483186414Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT28360.10833673318144797No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA28070.10722891750364053Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC27790.1061593023664471No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG27770.106082901285219No Hit
CAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACT27180.10382906938898999Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGTGTGAACGGACACTGAAATATTTTCTAGGAAGATCGGAAGAGCACA27060.10337066290162138No Hit
TGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGTCTGA26970.10302685803609492Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGAGCA26280.10039102073372541No Hit
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC26240.10023821857126922No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)