FastQCFastQC Report
Fri 14 Jul 2023
SRR9691485_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691485_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2613767
Total Bases198.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGC521361.9946689968922249No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA343091.3126265654130609No Hit
TGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGT330911.2660271554427No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA290961.113182621098208No Hit
TGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACAC278241.06451722743458No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC229780.8791143204424877No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA204740.7833138914065408Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCAC193410.7399664928052118No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG190980.7306695661855093No Hit
TGGTTGTGCAGCCAGATGCTTGGACAGAGGACAAGATCGGAAGAGCACAC179140.6853709607627612No Hit
TTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACA159520.610306886574052No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG157230.6015455853563075No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA137760.5270553955268392No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC114630.43856242733189305Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGA106400.4070753054882092No Hit
TGGTTGTGCAGCCAGATGCTTGGACAGAGGACAATGGCAGATCGGAAGAG101540.3884814522488041No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA96600.36958152735113725Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC94360.3610115209198066Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC85100.32558372647600187No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC82520.3157129154970585Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC80820.30920889275899494Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG80370.3074872396812723No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA75220.2877838766806682Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGTTGTGCAGCCAGATGCTTGGACAGAGGACAATGGCTAGATCGGAAGA70080.2681187726373468No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA63710.24374781684825003Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGTCT59540.2277938316613531Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTTGTGTGTGAACGGACACTGAAATATTTTCAGATCGGAAGAGCACAC57660.22060114769220057No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG53760.2056801543519373No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG52400.20047693616148649No Hit
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG48900.18708630111253222No Hit
AGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAGCACACGTCTG47600.1821126366657778Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA46320.1772154901335888Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTCACATCTCGTATG46120.1764503109879343TruSeq Adapter, Index 27 (97% over 37bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA44920.1718592361140071No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT44750.17120883384020075No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA42900.16413092674289637No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA42230.1615675766049537No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT41540.15892770855244556No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC40900.15647913528635107Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG37600.14385367938305135No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA36860.14102251654412962No Hit
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA34720.13283509968562615No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT34040.13023349059040074Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG31730.12139567145809094No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA30380.11623071222492287Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA30110.11519772037828926No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT29180.1116396373509957No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA29160.11156311943643026Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA28930.11068316341892755No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG28490.10899976929848758No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA28090.10746941100717852Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGAGCA27480.10513561461293222No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC27410.10486780191195313No Hit
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT27400.10482954295467041TruSeq Adapter, Index 3 (100% over 35bp)
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG27100.10368177423618861No Hit
TGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGTCTGA27030.10341396153520953Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGTGTGAACGGACACTGAAATATTTTCTAGGAAGATCGGAAGAGCACA26540.10153927262835592No Hit
CAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACT26350.10081235243998414Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)