FastQCFastQC Report
Fri 14 Jul 2023
SRR9691486_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691486_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2738539
Total Bases208.1 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGC559842.0443017243866164No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA354931.2960560357183155No Hit
TGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGT346151.2639951448564362No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA308491.1264765628680111No Hit
TGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACAC289861.0584475882943423No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC246610.9005166623517138No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA213160.7783712410157387Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG205500.7504001221089055No Hit
TTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCAC204030.7450322964179075No Hit
TGGTTGTGCAGCCAGATGCTTGGACAGAGGACAAGATCGGAAGAGCACAC188560.6885423212888332No Hit
TTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACA169700.619673482831539No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG160040.5843991997192663No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA145970.5330214395339996No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC122370.446844101909814Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGA110000.40167403129917084No Hit
TGGTTGTGCAGCCAGATGCTTGGACAGAGGACAATGGCAGATCGGAAGAG107420.39225294947415396No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA102620.3747253553810992Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC97980.3577820144244796Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC90520.33054121193819036No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC89460.3266705349093075Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG86020.3141090924759516No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC85020.3104575103732319Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA80430.2936967485217483Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGTTGTGCAGCCAGATGCTTGGACAGAGGACAATGGCTAGATCGGAAGA75590.27602309114458473No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA66200.24173473520004646Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGTCT63020.23012270411339766Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTTGTGTGTGAACGGACACTGAAATATTTTCAGATCGGAAGAGCACAC60860.22223528677152307No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG55510.20269932252197248No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG54820.20017973087109586No Hit
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG51550.18823905739520233No Hit
AGTTTGTGTGTGAACGGACACTGAAATAAGATCGGAAGAGCACACGTCTG51300.1873261618695224Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA49490.18071679826359968Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA49000.178927523033267No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT48490.17706521616087995No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTCACATCTCGTATG47780.17447259286794894TruSeq Adapter, Index 27 (97% over 37bp)
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA45620.16658517552607432No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC45390.16574531164244877Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT42860.15650680892256785No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA42420.15490011279737115No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG40610.14829074919144844No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA39180.14306898678455923No Hit
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA38000.13876011990334994No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT35140.12831659508957147Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG33720.12313134850370946No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT32060.1170697222131947No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA31410.11469619384642687Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA31340.1144405830992365No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG29900.10918230487132008No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA29780.10874411501899371No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA29270.10688180814660664Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC28950.10571330187373633No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA28910.10556723858962754Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGTCTGA28660.1046543430639476Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT28510.10410660574853964TruSeq Adapter, Index 3 (100% over 35bp)
AGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGAGCA27900.10187914066588061No Hit
ATTACTGAAGAGACTACTCATGTTGTTATGAAAACAGAGATCGGAAGAGC27730.10125837170841824No Hit
TGTGTGTGAACGGACACTGAAATATTTTCTAGGAAGATCGGAAGAGCACA27450.10023592871965673No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)