FastQCFastQC Report
Fri 14 Jul 2023
SRR9691488_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691488_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3258911
Total Bases247.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA800232.4555135135632735No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA749222.2989888340000695Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGC362391.1119972285220432No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA344591.0573777559436266No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG262530.8055758503377355No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA252830.7758113062921939No Hit
TGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACAC240160.7369332884512648No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC234210.7186756557635356No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA226980.6964903306656732No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA223020.6843390322718232Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGT215840.6623071326587318No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG186110.5710803394139944No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA166750.5116739917107279Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCAC163870.5028366837879279No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA163340.5012103736493572Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACA140840.43216890550248227No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG135330.41526141708073644No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG108680.33348563369788253No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA107580.3301102730329242Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC106080.3255075084897992Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC100250.30761809696552006No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG90670.2782217740834285Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCACATCTCGTATG88740.2722995503712743TruSeq Adapter, Index 11 (97% over 49bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG88140.27045844455402435No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA83110.25502384078607854No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC82020.2516791652180744Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGTTGTGCAGCCAGATGCTTGGACAGAGGACAAGATCGGAAGAGCACAC80310.2464320136389119No Hit
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG80010.2455114607302869No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA78220.24001882837549107No Hit
AGAGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGA77980.2392823860485911No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA77300.23719579945570773No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT68630.2105918203964453No Hit
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA67890.20832112322183696No Hit
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA64360.19748928399701618No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA62800.1927024088721662No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC61830.18972595446761203Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG60400.1853379856031662No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA57990.17794287723721208No Hit
AGTTATGCGTCTGGATCGTCTTACTAGATCGGAAGAGCACACGTCTGAAC56040.17195928333114957Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG53640.1645948600621496No Hit
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG52450.16094333352460377No Hit
AGGACGGGGATCAAGAGAGGTCAGATCGGAAGAGCACACGTCTGAACTCC50650.15542001607285377Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGA49730.15259698715307046No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC49540.1520139703109413Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA49130.15075588133582046Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGTTGTGCAGCCAGATGCTTGGACAGAGGACAATGGCAGATCGGAAGAG48570.14903751590638714No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAGATCGGAA48220.1479635375129913No Hit
AGTTTGTGTGTGAACGGACACTGAAATATTTTCAGATCGGAAGAGCACAC47560.1459383211140163No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG47260.1450177682053913No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC46090.14142761186175382No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA45640.1400467824988163No Hit
TGGTTGTGCAGCCAGATGCTTGGACAGAGGACAATGGCTAGATCGGAAGA45310.13903417429932882No Hit
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG44740.13728512377294133No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC40540.12439738305219136No Hit
TGTGGGCACTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC40300.12366094072529135TruSeq Adapter, Index 11 (100% over 36bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT40070.12295518349534552No Hit
TAAAGACTGACTAAGCATGTAGTACCGAGGATGAGATCGGAAGAGCACAC40040.12286312820448304No Hit
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA39990.12270970271971221No Hit
ATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATC38850.1192116016669372No Hit
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA37610.11540664964462055No Hit
TGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGTCT37420.11482363280249139Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGACTGACTAAGCATGTAGTACCGAGGATGAGATCGGAAGAGCACACGT37140.11396445008777473No Hit
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG37030.11362691402127889TruSeq Adapter, Index 11 (100% over 35bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT34570.1060783801705539Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA34510.1058942695888289No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACG33460.1026723344086414No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC33390.10245753872996224No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGA32630.1001254713614456No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)