FastQCFastQC Report
Fri 14 Jul 2023
SRR9691490_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691490_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3260403
Total Bases247.7 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA787292.414701495489975No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA727932.232638112527807Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGC348391.0685488879748914No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA336361.0316516087121745No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG266220.8165248283724434No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA244660.7503980336173166No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC235040.7208924786291756No Hit
TGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACAC235010.720800465463932No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA227810.698717305805448No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA218650.6706226193510434Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGT213220.6539682364419368No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG187110.5738861116248513No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA163560.50165577690856Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCAC159040.4877924600118452No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA157350.48260905170311763Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACA137880.4228925074599673No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG128710.39476714995048157No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA108450.33262759235591427Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG105370.32318090739089617No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC102150.3133048276547408Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC98440.3019258662196054No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG89950.275886140455643No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCACATCTCGTATG87720.26904649517252927TruSeq Adapter, Index 11 (97% over 49bp)
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG85850.2633110078723397Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA83930.257422165296744No Hit
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG79690.24441763794230345No Hit
TGGTTGTGCAGCCAGATGCTTGGACAGAGGACAAGATCGGAAGAGCACAC79150.24276140096791718No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC78070.23944892701914458Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGA77490.23767000582443337No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA75270.2308610315964008No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA75050.2301862683846138No Hit
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA69890.21436000396270033No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT69120.2119983327214458No Hit
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA66630.2043612400062201No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA63860.19586535774872002No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG60700.18617330434305207No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC60230.18473176475423436Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA57690.17694131676360253No Hit
AGTTATGCGTCTGGATCGTCTTACTAGATCGGAAGAGCACACGTCTGAAC54850.16823073712053388Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG54730.16786268445955915No Hit
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG52580.16126840761709518No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC49860.15292588063500126Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGA49470.15172970948683337No Hit
AGGACGGGGATCAAGAGAGGTCAGATCGGAAGAGCACACGTCTGAACTCC48430.14853991975838568Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA48170.1477424723262738Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC47220.14482872209355713No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAGATCGGAA47050.1443073141571763No Hit
AGTTTGTGTGTGAACGGACACTGAAATATTTTCAGATCGGAAGAGCACAC45920.14084148493299756No Hit
TGGTTGTGCAGCCAGATGCTTGGACAGAGGACAATGGCAGATCGGAAGAG45910.14081081387791633No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA45900.1407801428228351No Hit
TGGTTGTGCAGCCAGATGCTTGGACAGAGGACAATGGCTAGATCGGAAGA44220.13562740556918884No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG43760.13421653703545236No Hit
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG42990.13185486579419783No Hit
ATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATC39290.12050657541414359No Hit
TAAAGACTGACTAAGCATGTAGTACCGAGGATGAGATCGGAAGAGCACAC38520.11814490417288906No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC38480.11802221995256415No Hit
TGTGGGCACTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC38170.11707141724504608TruSeq Adapter, Index 11 (100% over 36bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT38080.11679537774931503No Hit
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA38010.11658068036374644No Hit
TGTGAACGGACACTGAAATATTTTCTAGGAGATCGGAAGAGCACACGTCT37920.1163046408680154Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG37390.11467907494871032TruSeq Adapter, Index 11 (100% over 35bp)
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA37230.11418833806741069No Hit
AAGACTGACTAAGCATGTAGTACCGAGGATGAGATCGGAAGAGCACACGT35940.11023177196193232No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC34470.10572312686499183No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA34080.10452695571682397No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT33770.10357615300930591Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACG32980.10115313965788893No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA32870.10081575805199541Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)