FastQCFastQC Report
Fri 14 Jul 2023
SRR9691491_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691491_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5446158
Total Bases413.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT2162543.9707625081754885No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC926241.7007218666810622No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA810951.489031350173829No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG720261.322510290740739No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA709081.301982057810295Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCGGAAGAGCACA692311.2711897084146293No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG610631.1212124216741417No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG607381.1152449121013381No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC552941.0152845363649017No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA464770.8533905920467235Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA463350.8507832494026065No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC440520.8088637898496518No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTAGATCGGAAGAGCACAC355790.6532862248946872No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT299090.5491761348091627No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGAGATCGGAAGAGCACACGTCT284440.5222764378117565Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA267460.4910984954898481No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC252100.4628951271703832Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA247990.45534852275677645TruSeq Adapter, Index 7 (100% over 35bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACAGATCGGAAGAGCACACG241650.4437072886978306No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA227060.41691776110792234No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCAGATCGGAAGAGCACACGT222810.40911409474348703No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGAAGATCGGAAGAGCACACG219020.40215506050320243No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAAGATCGGAAGAGCACACGT218520.4012369821073865No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC213220.39150535111173784No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGAGATCGGAAGAGCA206660.3794601625586331No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTAGATCGGAAGAGCACA179040.32874551197376206No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC160530.29475824976065695Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCAGATCGGAAGAGCACACGTCTG158360.2907737895228159Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCGCGTTAACAAAGCGGTTATGTAGCGGATTAGATCGGAAGAGCACACG149650.27478086386770273No Hit
CACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGAGATCGGAAGAGC149130.2738260623360541No Hit
GGGTCGTTAGCTCAGTTGGTAGAGCAGATCGGAAGAGCACACGTCTGAAC142130.2609729647946314Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC140500.2579800292242715No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA122860.2255902234198861No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG120170.2206509616503965Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC119730.21984305266207846No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA119530.2194758213037521No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC117970.21661141670880646No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG117680.21607893123923322No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC117050.2149221524605052Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATAGATCGGAAGAGCACAC116600.21409588190427087No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG115010.21117639260557625No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGAGATCGGAAGAGCACACGTCTGAA113370.20816509546730008Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT113180.20781622567689Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACAGATCGGAAGAGCACACG109000.20014109028786897No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC108910.1999758361766221No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCAGATCGGAAGAGCACACGT107820.19797442527374343No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG91670.16832049308888947No Hit
AGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCA82740.15192361293961726Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTAGATCGGAAGAGCACA79460.14590101866306487No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC78750.14459734734100627Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCAGATCGGAAGAGCAC74110.1360775798278346No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA69940.1284208060067299Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69040.12676826489426124No Hit
GCGGGCGTAGTTCAATGGTAGAACGAGAGCAGATCGGAAGAGCACACGTC68410.1256114861155332No Hit
AGCGTGAGCGGTAATTGTCCGCTGAACCTGGATGAGATCGGAAGAGCACA68270.12535442416470474No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA66410.12193917253226953Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTGTATCTCGTATG63070.11580640884821924TruSeq Adapter, Index 7 (97% over 35bp)
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC63000.11567787787280502Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAAGATCGGAAGAGCAC61390.11272166543827777No Hit
ACGGACGTGGGGGTTCAAGTCCCAGATCGGAAGAGCACACGTCTGAACTC61310.11257477289494723Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTAGATCGGAAGAGCACAC60870.11176686390662924No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT60710.11147307881996812No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG60490.11106912432580912No Hit
AGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCAGATCGGAAGAGCACA59710.1096369220283363No Hit
CAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTAGATCGGAAGAG59300.10888409774376727No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG58870.10809455032336557No Hit
TTCGTTGTCTGACACCGGAAGATGAGATCGGAAGAGCACACGTCTGAACT57720.10598297001298898Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC57170.10497308377759146No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC56270.10332054266512282No Hit
ACGGACGTGGGGGTTCAAGTCCCCCAGATCGGAAGAGCACACGTCTGAAC55090.10115387765099726Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)