FastQCFastQC Report
Fri 14 Jul 2023
SRR9691494_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691494_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5424276
Total Bases412.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT2179104.017310328604222No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC923201.7019782916650996No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA815501.5034264480642208No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG719501.3264443033503457No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA700951.2922461910124041Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCGGAAGAGCACA685351.2634865924963994No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG609481.123615391252215No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG606671.1184349763913193No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC550431.0147529366131074No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA464000.8554136994503967No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA458400.8450897410087539Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC437750.8070201442551964No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTAGATCGGAAGAGCACAC355390.655184212602751No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT300530.5540462911548011No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGAGATCGGAAGAGCACACGTCT280140.516456021043177Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA267830.4937617481116374No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC257220.4742015339927393Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA251640.46391444683124533TruSeq Adapter, Index 7 (100% over 35bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACAGATCGGAAGAGCACACG240600.4435615001891497No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA226650.4178437822854147No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCAGATCGGAAGAGCACACGT223240.41155722902005726No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGAAGATCGGAAGAGCACACG220390.40630307159886403No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAAGATCGGAAGAGCACACGT217460.40090142905707604No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC213920.39437521247075186No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGAGATCGGAAGAGCA203770.37566303779527443No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATTAGATCGGAAGAGCACA178890.32979516529026176No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC160450.2957998449931382Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCAGATCGGAAGAGCACACGTCTG158250.2917440041767786Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCGCGTTAACAAAGCGGTTATGTAGCGGATTAGATCGGAAGAGCACACG148690.2741195322656885No Hit
CACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGAGATCGGAAGAGC148060.27295808694100376No Hit
GGGTCGTTAGCTCAGTTGGTAGAGCAGATCGGAAGAGCACACGTCTGAAC143840.26517824682962293Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC143350.26427490046597923No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA124010.22862037256216314No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC123040.22683211547495002No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA120370.22190979957509538No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC119980.22119080961219528Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC118310.2181120577197768No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGATAGATCGGAAGAGCACAC117200.21606570167152261No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT116750.21523609786817632Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG116490.21475677122624293Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG116040.21392716742289664No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG114980.21197298957501423No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGAGATCGGAAGAGCACACGTCTGAA109660.20216522905545367Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCAGATCGGAAGAGCACACGT109300.20150154601277664No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACAGATCGGAAGAGCACACG109050.20104065501091758No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC108070.19923396228363013No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG92960.17137771013126915No Hit
AGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCA81500.15025046660605026Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTAGATCGGAAGAGCACA79490.14654490295110353No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78920.14549407146686488No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC78810.14529127942604692Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCAGATCGGAAGAGCAC74880.13804607287682263No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA69010.12722435215317213Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGTGAGCGGTAATTGTCCGCTGAACCTGGATGAGATCGGAAGAGCACA68460.12621039194908223No Hit
GCGGGCGTAGTTCAATGGTAGAACGAGAGCAGATCGGAAGAGCACACGTC67010.12353722413829976No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC64270.11848585875792457Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA64040.11806183903621424Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG62040.11437471102134183No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGACTAGATCGGAAGAGCACAC61340.1130842162161365No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTGTATCTCGTATG61200.11282611725509542TruSeq Adapter, Index 7 (97% over 35bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAAGATCGGAAGAGCAC60780.11205182037197223No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT60650.11181215705100551No Hit
ACGGACGTGGGGGTTCAAGTCCCAGATCGGAAGAGCACACGTCTGAACTC60190.11096411760758487Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTAGATCGGAAGAG60150.11089037504728742No Hit
AGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCAGATCGGAAGAGCACA59600.10987641484319752No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG58820.10843843491739728No Hit
TTCGTTGTCTGACACCGGAAGATGAGATCGGAAGAGCACACGTCTGAACT56620.10438259410103763Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC56560.10427198026059145No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC55880.10301835673553485No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)