FastQCFastQC Report
Fri 14 Jul 2023
SRR9691496_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691496_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3604261
Total Bases273.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA836082.319698823142941Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG526071.459577982837536No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA463871.2870044649929624No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC410951.140178250132274No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA343250.9523450160795791No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT332080.9213539197078124TruSeq Adapter, Index 1 (100% over 35bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA308810.8567914476781787Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA225590.6258980689800212Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA193810.5377246542356394Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA183490.5090918776414916No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG156830.4351238714399429No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG108770.3017816967195217No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA104250.2892409844903019No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC101930.2828041587443307Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC97670.2709848149176766No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA92730.25727881526892754Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT89090.24717965763300714No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG88160.24459937834690662Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG81590.22637095371284155No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG75080.20830899871013783No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG74380.20636685301092234No Hit
TCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAAC71050.19712778847036883Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT70580.19582377635803844No Hit
TCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGAA64150.17798378086381647Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGACTGACTAAGCATGTAGTACCGAGGAGATCGGAAGAGCACACGTCTG63640.1765687889972452Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGACGTGGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT62650.17382204007978336TruSeq Adapter, Index 1 (100% over 35bp)
TTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCAGATCGG61870.1716579348720861No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61120.16957706448006957No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC60900.1689666758317447Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT60830.16877246126182316Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA59530.16516561924899445No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA59010.16372288244386296No Hit
CGGACGTGGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT58990.16366739256674254TruSeq Adapter, Index 27 (100% over 36bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG58260.16164201205184642No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC55320.15348500011514152Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA55210.15317980579097906Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT54670.15168157910872712Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC54640.15159834429304647No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC54460.15109893539896252Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT51330.14241476962961339No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG51240.1421650651825714No Hit
TTGAAGGTCGTCTGGTTGCATTTATCAGATCGGAAGAGCACACGTCTGAA47950.13303698039625875Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC47370.13142777395976596No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGGCATCTCGTATG46410.12876425985798476TruSeq Adapter, Index 12 (97% over 37bp)
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG46340.1285700452880632No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA43250.11999685927295498Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA41750.11583511848892186No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC41130.11411493229818816No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAGATCGGAA38840.10776134136789761No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT38270.10617987986996501Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTGCGTCGCGAAACTCTGGAAGATGCAGATCGGAAGAGCACACGTCTG37290.10346087589106338Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCTG37260.10337764107538272Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGAGCACACGTC36470.10118579092912529No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)