FastQCFastQC Report
Fri 14 Jul 2023
SRR9691497_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691497_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3737249
Total Bases284 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA856592.2920335251946016Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG541751.4495956785325248No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA474521.2697039988504915No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC427301.1433543764410667No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA358670.9597166257854374No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT345870.925466834026847TruSeq Adapter, Index 1 (100% over 35bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA323280.8650213031028973Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA227720.6093252015051713Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA198560.5312998946551327Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA191890.5134525422309297No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG161350.43173467970691815No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA114770.30709754688542296No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG109860.29395954082802617No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC106300.2844338174951683Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC105320.2818115678136512No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA94160.2519500306241302Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT91550.24496628402335516No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG89400.23921338931390443Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG83480.2233728606255564No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG77410.20713096719003737No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG76810.20552550820135346No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT73490.19664196846396909No Hit
TCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAAC72280.19340429283678984Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGACTGACTAAGCATGTAGTACCGAGGAGATCGGAAGAGCACACGTCTG65280.17467393796881076Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGAA65160.17435284617107397Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGACGTGGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT65060.17408526967295998TruSeq Adapter, Index 1 (100% over 35bp)
TTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCAGATCGG64710.17314875192956103No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT63290.16934916565634242Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC62680.1677169490178471Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA62410.16699449247293932No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA60810.16271326850311552No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG60800.16268651085330413No Hit
CGGACGTGGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT58460.15642522079743684TruSeq Adapter, Index 27 (100% over 36bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC56750.15184966267968766Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC55510.1485317141030742Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC55340.14807683405628044No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT55080.1473811351611841Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA54840.1467389515657105Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG53360.1427788193936235No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT53080.1420296051989043No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC49900.13352067255887953No Hit
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG47280.12651016830829306No Hit
TTGAAGGTCGTCTGGTTGCATTTATCAGATCGGAAGAGCACACGTCTGAA46610.12471740577092937Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA45670.12220218668865789Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGGCATCTCGTATG44840.11998130175431179TruSeq Adapter, Index 12 (97% over 37bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC43320.1159141389829792No Hit
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA42410.1134791928501419No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAGATCGGAA40940.10954581832786629No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT39600.10596029325313887Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTGCGTCGCGAAACTCTGGAAGATGCAGATCGGAAGAGCACACGTCTG38750.10368589301916999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC37860.1013044621859555Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)