FastQCFastQC Report
Fri 14 Jul 2023
SRR9691499_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691499_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3002376
Total Bases228.1 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG334951.1156164317860255No Hit
ATTGGACCGATGCAAAATGTAGCCAGATCGGAAGAGCACACGTCTGAACT321791.0717844800251535Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA273690.9115780302000815No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC262370.8738745580167174Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA260100.8663138794075093Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG227230.7568339208680058No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC222350.7405801272059196TruSeq Adapter, Index 6 (100% over 35bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC221540.7378822639136471No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG178860.5957281832788431Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT140300.46729656778498097No Hit
TGATGTTCGTTGTCGTCGCACCGGAGATCGGAAGAGCACACGTCTGAACT135940.4527747357426252Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGATTGGACCGATGCAAAATGTAGCCAGATCGGAAGAGCACACGTCTGA118000.3930220598619227Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCAGGGTCTGCAGGATGTTTGGCGAGATCGGAAGAGCACACGTCTGAAC112690.37533606716813617Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA101740.33886495229111874No Hit
ATGCAGGGTCTGCAGGATGTTTGGCGAGATCGGAAGAGCACACGTCTGAA83010.27648102702659494Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT81440.2712518352131778Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC80950.2696197944561241Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA77250.257296221392657Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC74470.24803688811794392No Hit
ATGGTCGTGGTCCGCTGACCACCCTGCTGTATAGTCTGAGATCGGAAGAG65980.21975928398042083No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA65560.2183603919029462No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA65080.21676165810011805Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA63530.2115990801951521No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC63330.21093294111064037Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTGGACCGATGCAAAATGTAGCCATCTGCTGCAACGAGATCGGAAGAGC62320.20756893873385612No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTAAATCTCGTATG61020.20323903468452983TruSeq Adapter, Index 6 (97% over 38bp)
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA59340.19764346637463132Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC58580.1951121378534867Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTTCTGGTGTAGATCTGGGGACCGAGAACCTGTACTAGATCGGAAGAG57380.19111530334641633No Hit
ATCTGGGGACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGA53730.17895826505407716Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG51910.1728963993850204No Hit
TCTTCTGGTGTAGATCTGGGGACCGAGAACCTGTACTAGATCGGAAGAGC51540.17166404207867367No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC49990.16650146417370776No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCGGAAGAGCACA49310.16423659128636786No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC46090.15351175202572898No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT44390.1478495698073792No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG44110.1469169750890628No Hit
ATTGGACCGATGCAAAATGTAGCCATCTGCAGATCGGAAGAGCACACGTC43540.14501847869820436No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT43160.14375281443763205Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGACTGACTAAGCATGTAGTACCGAGGAGATCGGAAGAGCACACGTCTG43000.14321990317002267Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGGACCGATGCAAAATGTAGCCATCTGCTGCAAGATCGGAAGAGCACA42890.14285352667354123No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT42280.14082180246578044Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG42220.14062196074042693No Hit
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG42090.14018897033549427Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG36730.12233644287057983No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG35480.1181730735923815Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA34380.11450930862756696Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG34260.11410962517685994Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTGGACCGATGCAAAATGTAGCCATCTGCTGCAGATCGGAAGAGCACAC33960.11311041655009232No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGAGATCGGAAGAGCA33750.11241097051135501No Hit
ACGGACGTGGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC33550.11174483142684327TruSeq Adapter, Index 6 (100% over 35bp)
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA30890.10288518160283722Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG30620.10198589383874639No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG30500.10158621038803935No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT30450.10141967561691141No Hit
CGGACGTGGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC30400.10125314084578348TruSeq Adapter, Index 6 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)