FastQCFastQC Report
Fri 14 Jul 2023
SRR9691503_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691503_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3827680
Total Bases290.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG453471.184712410650838No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC427391.1165771433348661No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA372140.972233833549304No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG366970.9587269573214062No Hit
ATTGGACCGATGCAAAATGTAGCCAGATCGGAAGAGCACACGTCTGAACT344890.9010418843790495Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA273020.7132780169711157No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC258170.6744816703590686Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA246350.6436013459850353Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGATTGGACCGATGCAAAATGTAGCCAGATCGGAAGAGCACACGTCTGA163800.42793545959954854Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGATGTTCGTTGTCGTCGCACCGGAGATCGGAAGAGCACACGTCTGAACT139570.36463340718137355Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTTCTGGTGTAGATCTGGGGACCGAGAACCTGTACTAGATCGGAAGAG137320.35875517284621494No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA136510.35663900848555785No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT134740.35201479747523307No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG133130.3478085942398529TruSeq Adapter, Index 4 (100% over 35bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT119810.31300944697571376Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA116740.304988922793964Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGTGTAGATCTGGGGACCGAGAACCTGTACTAGATCGGAAGAGC110490.28866049408519No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC108500.2834615223843164No Hit
TGCAGGGTCTGCAGGATGTTTGGCGAGATCGGAAGAGCACACGTCTGAAC101300.26465117251180875Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGTCGTGGTCCGCTGACCACCCTGCTGTATAGTCTGAGATCGGAAGAG96280.25153617857292143No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA89370.23348346779250095No Hit
ATGCAGGGTCTGCAGGATGTTTGGCGAGATCGGAAGAGCACACGTCTGAA82920.21663252936504618Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGGACCGATGCAAAATGTAGCCATCTGCTGCAACGAGATCGGAAGAGC80000.20900388747230697No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC79430.20751473477406682No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA76390.19957258705011913Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTGGGGACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGA73370.19168269029803955Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCTTCTGGTGTAGATCTGGGGACCGAGAACCTGTACTAGATCGGAAGA71880.18778999289386783No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG66750.17438761860970614No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT62490.16325816160180578No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT61690.16116812272708272No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA60340.15764118212598754Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT57620.1505350499519291Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG56810.148418885591272No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT56100.14656397608995528Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGGACCGATGCAAAATGTAGCCATCTGCAGATCGGAAGAGCACACGTC54830.1432460393763324No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG53980.14102537307193913No Hit
TCTGGTGTAGATCTGGGGACCGAGAACCTGTACTAGATCGGAAGAGCACA53740.14039836140952222No Hit
ATTGGACCGATGCAAAATGTAGCCATCTGCTGCAAGATCGGAAGAGCACA52810.13796869121765665No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC51560.13470300547590183Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG47250.12344292103833132No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG46640.12184926639635497No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC46360.12111775279020189Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTGGTGTAGATCTGGGGACCGAGAACCTGTACTAGATCGGAAGAGCAC43620.11395936964427539No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA43180.11280984826317769No Hit
ATTGGACCGATGCAAAATGTAGCCATCTGCTGCAGATCGGAAGAGCACAC42700.11155582493834386No Hit
AAGACTGACTAAGCATGTAGTACCGAGGAGATCGGAAGAGCACACGTCTG42440.11087656230405886Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG41360.10805500982318272No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCGGAAGAGCACA39500.10319566943945158No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC39460.10309116749571542Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT39380.10288216360824312Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGGGGACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGAA38550.10071374827571793Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)