FastQCFastQC Report
Fri 14 Jul 2023
SRR9691504_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691504_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3840817
Total Bases291.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG455021.1846958602818098No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC433451.1285359338911487No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA371130.9662787891222102No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG370350.9642479711998775No Hit
ATTGGACCGATGCAAAATGTAGCCAGATCGGAAGAGCACACGTCTGAACT343940.8954865592398701Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA269890.7026890372543133No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC256980.6690763970269866Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA245660.6396035010259535Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGATTGGACCGATGCAAAATGTAGCCAGATCGGAAGAGCACACGTCTGA167530.4361832391389644Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGATGTTCGTTGTCGTCGCACCGGAGATCGGAAGAGCACACGTCTGAACT144250.37557113499549705Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTTCTGGTGTAGATCTGGGGACCGAGAACCTGTACTAGATCGGAAGAG140830.3666667794898846No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA138090.35953288063451083No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT137340.35758017109380635No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG134010.3489101407330784TruSeq Adapter, Index 4 (100% over 35bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT120290.3131885742017909Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA117650.3063150366185111Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGTGTAGATCTGGGGACCGAGAACCTGTACTAGATCGGAAGAGC112050.2917348053812509No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC109850.28600685739518444No Hit
TGCAGGGTCTGCAGGATGTTTGGCGAGATCGGAAGAGCACACGTCTGAAC102240.2661933645888362Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGTCGTGGTCCGCTGACCACCCTGCTGTATAGTCTGAGATCGGAAGAG95720.24921780964831183No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA90330.23518433708244885No Hit
ATGCAGGGTCTGCAGGATGTTTGGCGAGATCGGAAGAGCACACGTCTGAA82670.21524066364005368Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGGACCGATGCAAAATGTAGCCATCTGCTGCAACGAGATCGGAAGAGC80890.21060623299678166No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC79340.20657063327932573No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA77780.20250899743466036Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTGGGGACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGA74970.1951928456888209Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCTTCTGGTGTAGATCTGGGGACCGAGAACCTGTACTAGATCGGAAGA71570.1863405624376272No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG64460.16782887599174864No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT62180.161892638988007No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA62090.16165831384312243Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT61280.1595493875391616No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG59470.15483684851426144No Hit
TCTGGTGTAGATCTGGGGACCGAGAACCTGTACTAGATCGGAAGAGCACA56220.14637510717120863No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT56010.14582834849981136Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT55350.14410996410399143Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGGACCGATGCAAAATGTAGCCATCTGCAGATCGGAAGAGCACACGTC54600.14215725456328693No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG54470.14181878490956482No Hit
ATTGGACCGATGCAAAATGTAGCCATCTGCTGCAAGATCGGAAGAGCACA53350.13890273866211278No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC51570.1342683080188408Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG49540.12898297419533397No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG47030.12244790626577627No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC46460.12096384701484085Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTGGTGTAGATCTGGGGACCGAGAACCTGTACTAGATCGGAAGAGCAC43770.11396012879551407No Hit
ATTGGACCGATGCAAAATGTAGCCATCTGCTGCAGATCGGAAGAGCACAC43530.11333526174248865No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG43090.11218967214527535No Hit
AAGACTGACTAAGCATGTAGTACCGAGGAGATCGGAAGAGCACACGTCTG42920.11174705798271566Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA42880.11164291347387809No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT39780.1035717140389662Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC39250.10219179929686834Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCGGAAGAGCACA39230.10213972704244956No Hit
TCTGGGGACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGAA38490.10021305362895447Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)