FastQCFastQC Report
Fri 14 Jul 2023
SRR9691506_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691506_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3642522
Total Bases276.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG443551.2177002637183798No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC413821.1360809900393187No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA357600.9817373786623663No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG352100.9666379502992708No Hit
ATTGGACCGATGCAAAATGTAGCCAGATCGGAAGAGCACACGTCTGAACT330420.9071187490425591Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA261820.7187876970955838No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC250820.6885888403693924Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA238400.6544915857749108Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGATTGGACCGATGCAAAATGTAGCCAGATCGGAAGAGCACACGTCTGA158790.43593422359562967Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGATGTTCGTTGTCGTCGCACCGGAGATCGGAAGAGCACACGTCTGAACT139230.3822351656352384Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTTCTGGTGTAGATCTGGGGACCGAGAACCTGTACTAGATCGGAAGAG134410.36900257568794365No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA130550.358405522327662No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG129770.3562641488507139TruSeq Adapter, Index 4 (100% over 35bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT129560.35568762522230474No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT115800.3179116008084508Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA111950.3073420009542839Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGTGTAGATCTGGGGACCGAGAACCTGTACTAGATCGGAAGAGC111130.3050908134528769No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC103730.2847752189279845No Hit
TGCAGGGTCTGCAGGATGTTTGGCGAGATCGGAAGAGCACACGTCTGAAC100200.27508413126948855Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGTCGTGGTCCGCTGACCACCCTGCTGTATAGTCTGAGATCGGAAGAG91860.25218790716981254No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA83820.23011528825357813No Hit
ATGCAGGGTCTGCAGGATGTTTGGCGAGATCGGAAGAGCACACGTCTGAA77910.21389026613977896Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGGACCGATGCAAAATGTAGCCATCTGCTGCAACGAGATCGGAAGAGC77590.21301175394410793No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC77350.2123528697973547No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA73100.20068512969859892Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTGGGGACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGA70760.1942610092677546Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCTTCTGGTGTAGATCTGGGGACCGAGAACCTGTACTAGATCGGAAGA70100.1924490778641831No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67820.1861896784700271No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG62780.1723531113882085No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT61380.16850962053214777No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA59390.1630463728153186Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT58260.15994412662435534No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG57370.15750076458014528No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT54150.14866073561120563Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGGTGTAGATCTGGGGACCGAGAACCTGTACTAGATCGGAAGAGCACA53700.14742532783604326No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG53240.14616246655476617No Hit
ATTGGACCGATGCAAAATGTAGCCATCTGCAGATCGGAAGAGCACACGTC53210.14608010603642202No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT52350.14371910451055614Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGGACCGATGCAAAATGTAGCCATCTGCTGCAAGATCGGAAGAGCACA51260.14072667234405173No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC50500.13864020587933307Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG46630.12801569901293663No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG44170.12126213650871566No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC43300.11887368147673508Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTGGTGTAGATCTGGGGACCGAGAACCTGTACTAGATCGGAAGAGCAC42550.1168146685181311No Hit
ATTGGACCGATGCAAAATGTAGCCATCTGCTGCAGATCGGAAGAGCACAC42160.11574398177965706No Hit
AAGACTGACTAAGCATGTAGTACCGAGGAGATCGGAAGAGCACACGTCTG41720.11453602751060941Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG41430.11373987583328256No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCGGAAGAGCACA38690.10621761515784943No Hit
TCTGGGGACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGAA38260.10503711439491649Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA37900.1040487881747866No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC37780.10371934610140995Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT37480.10289574091796837Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG37470.10286828741185366Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)