FastQCFastQC Report
Fri 14 Jul 2023
SRR9691507_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691507_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600013
Total Bases121.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA215121.3444890760262573No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC200571.2535523148874417No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG196641.2289900144561325No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA187521.1719904775773697No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG115690.7230566251649205No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA113860.711619218093853No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA95100.5943701707423628No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG73820.46137125135858276No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA71520.4469963681545087No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA69170.43230898748947666Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA68370.42730902811414656Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG67720.4232465611216909No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC66080.4129966444022642No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT57630.3601845735003403No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG56990.3561846060000763No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG56550.3534346283436447No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA55800.3487471664292728Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG47270.29543509958981584No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA44990.2811852153701251No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG35420.2213732013427391No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA30380.18987345727815963No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29420.18387350602776353No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA25330.1583112137213885No Hit
TCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGAA25090.1568112259087895Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC24240.1514987690725013No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC22820.1426238411812904No Hit
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG22380.13987386352485887No Hit
AAGACTGACTAAGCATGTAGTACCGAGGATGAGATCGGAAGAGCACACGT21960.13724888485281056No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGATATC19980.12487398539886864TruSeq Adapter, Index 12 (97% over 37bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC19060.11912403211723904Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTC17870.11168659254643556No Hit
TAAAGACTGACTAAGCATGTAGTACCGAGGATGAGATCGGAAGAGCACAC17670.11043660270260304No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA17430.10893661489000402Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGAGATCGG17160.10724912860083011No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAAGA17130.10706163012425524No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG16500.10312416211618282TruSeq Adapter, Index 2 (100% over 35bp)
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC16230.10143667582700891No Hit
AGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAGA16220.10137417633481727No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)