FastQCFastQC Report
Fri 14 Jul 2023
SRR9691513_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691513_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024363
Total Bases153.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA148730.7347002489178077No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC144950.7160277084692814No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG135690.6702849241959076No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA121360.5994972245590341Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA120410.5948043903193251No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG114230.5642762686336393No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA101420.5009971037803003No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA94700.4678014763162535Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG88120.43529742442437447No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA76490.37784725367930555Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC76290.3768592885762089No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA57500.2840399671402807No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG54130.26739275515310246No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATCGATC46680.23059105506275304TruSeq Adapter, Index 8 (97% over 37bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA43140.21310407273794274No Hit
TCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGAA43090.21285708146216858Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG42490.20989318615287872No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT42400.20944860185648523No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40020.19769181712963538No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA38090.1881579538847529Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT36400.17980964876358638No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG36210.17887108191564458No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG32280.15945756763979582No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC30960.15293699795935808TruSeq Adapter, Index 5 (100% over 35bp)
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA29100.14374892250055943Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA25850.12769448957523924No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA23980.11845701586128575No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT23480.11598710310354418Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGACTGACTAAGCATGTAGTACCGAGGATGAGATCGGAAGAGCACACGT22190.10961472818857092No Hit
AAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGAGATCGG21090.10418092012153947No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATCGATCTCGTATG20730.10240258293596555TruSeq Adapter, Index 8 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)