FastQCFastQC Report
Fri 14 Jul 2023
SRR9691515_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691515_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4461324
Total Bases339 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA799081.7911274769552714No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC726901.6293369412308991No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG686221.5381532477802555No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC505531.1331389515758101No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA502881.1271990108765917No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG421500.944786794234178No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA396730.8892651598494079Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC301530.675875592088806No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG280120.6278853542132335No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA246910.5534455690732168No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC232200.5204732944749138No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT178490.4000830246805657Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA164950.3697332899381439No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGATCTCGTATG149520.33514714465929846TruSeq Adapter, Index 9 (97% over 49bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA133660.29959715994624014No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG130660.29287269877731364Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGAACTCC120820.2708164661432346Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC101280.2270178090629598No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT99670.22340901490230256Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA96050.21529483175846453Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC95110.2131878339255342No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG91320.2046925979821237No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC78010.17485840526265298Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATAGCGTTCTGGATTGTAAACTGAGATCGGAAGAGCACACGTCTGAACTC75850.17001679322102586Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGAGATCGG75030.1681787738348526No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG73670.1651303514382726No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG71990.1613646531836737No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT66270.14854334722158713No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG66130.1482295390337039No Hit
AGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCA65640.14713121037611257Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC65140.14601046684795815Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG62120.13924117593790544Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGACATCTACACTCGTCTGGGTGGAGATCGGAAGAGCACACGTCTGAAC59510.13339089472093935Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACCAGATCGGAAGAGC57070.12792166630354576No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT55640.12471633981302412No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC55100.12350593680261734Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC54860.12296797990910321Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGGAAGAGCACACG52470.11761082584452508No Hit
TTCTGGATGGTGGTATTCCGTTTAACAAAGCAGATCGGAAGAGCACACGT52100.11678147563369082No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACCAGATCGGAAGAG51920.11637800796355521No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT49840.11171571488643281No Hit
ATGTTGGTGGTGGTACAGGTGCAACCCTGAATATGAGATCGGAAGAGCAC49550.11106568364010325No Hit
TCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGAA47610.10671719875086408Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA46440.10409465889498276No Hit
TCTGCGTCTGCTGGCAACCTATAGCGTTCAGATCGGAAGAGCACACGTCT45200.10131521494515978Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)