FastQCFastQC Report
Fri 14 Jul 2023
SRR9691518_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691518_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4951743
Total Bases376.3 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA871591.7601680862678053No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC813721.6433001470391335No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG769971.5549474195248016No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA558381.1276433369017738No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC554031.118858551423206No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG459910.9287840665398022No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA430720.8698351267422401Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC338460.6835168949600171No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG304700.6153388816826721No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA275820.5570159840686401No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC261460.5280160945347931No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT198050.3999601756391638Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA189890.3834811297759193No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGATCTCGTATG161740.32663246052955497TruSeq Adapter, Index 9 (97% over 49bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA144800.29242228443600565No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG138530.2797600764013803Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGAACTCC131130.2648158436332419Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC110350.2228508224275775No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT109130.2203870435117493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA107660.21741839186726775Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC104570.21117816494111266No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG100510.20297903182778265No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC89080.17989625067375267Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGAGATCGG84340.17032386373848563No Hit
ATAGCGTTCTGGATTGTAAACTGAGATCGGAAGAGCACACGTCTGAACTC82820.1672542375482734Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG81660.164911628087322No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG77860.15723756261179145No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT73120.1476651756765244No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG72670.14675640476494842No Hit
AGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCA71480.1443532105765586Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG70890.14316171093693675Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC67300.13591173855347502Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGACATCTACACTCGTCTGGGTGGAGATCGGAAGAGCACACGTCTGAAC65430.13213529054314813Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACCAGATCGGAAGAGC61470.12413810652127948No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC61380.12395635233896428Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC60690.1225629036078811Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT60660.12250231888044269No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACCAGATCGGAAGAG57980.11709008322927905No Hit
TGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGGAAGAGCACACG56950.11501000758722736No Hit
TTCTGGATGGTGGTATTCCGTTTAACAAAGCAGATCGGAAGAGCACACGT56350.11379831303845939No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT54270.10959777193606372No Hit
ATGTTGGTGGTGGTACAGGTGCAACCCTGAATATGAGATCGGAAGAGCAC54120.10929484829887172No Hit
TCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGAA54100.10925445848057946Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA53030.10709360320194325No Hit
TCTGCGTCTGCTGGCAACCTATAGCGTTCAGATCGGAAGAGCACACGTCT49540.10004557990994283Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)