FastQCFastQC Report
Fri 14 Jul 2023
SRR9691519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5319434
Total Bases404.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA1053671.9807934453176788Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC855161.6076146447159605No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG841111.5812020602191885No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA632581.189186669108029No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA469470.882556302042661No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG327430.6155354122261879No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC274280.5156187669590412No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT250370.4706703758332183Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG241080.4532061117780576No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG221240.41590891060966256Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCA179720.33785549364838435Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA179600.3376299057380917No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT176690.33215939891349344No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA167210.31433795400036924No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT163610.30757031669158785Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA151830.2854251034978533No Hit
ATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGAACTCC137610.258692936128167Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC135100.25397438900454444No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC123360.2319043717809075Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC122920.23107721610983425No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG119130.2239523979430894No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA117970.2217717148102599Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG112660.2117894497798074No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC106450.20011527542215957No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA104490.19643067288737862Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCATCATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTG102420.19253928143482935Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC101620.19103536203287794No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA97960.18415493076895023Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAAGTATCTCGTATG91510.17202958059071696TruSeq Adapter, Index 3 (97% over 36bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC89000.16731103346709444No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC88870.16706664656427733TruSeq Adapter, Index 5 (97% over 35bp)
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGAGATCGGAAGAGCACAC88840.16701024958670416No Hit
AGAATGTGGCGCTCCGCGGTTGAGATCGGAAGAGCACACGTCTGAACTCC88700.16674706369136266Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGTTGGTGGTGGTACAGGTGCAACCCTGAATATGAGATCGGAAGAGCAC87440.16437839063328918No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA82820.15569325608701978Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG82550.1551856832888612No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC80810.15191465858961686Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC78710.14796687015949442Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATAGCGTTCTGGATTGTAAACTGAGATCGGAAGAGCACACGTCTGAACTC78300.14719611146599432Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA76610.14401908172937194No Hit
ACGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC71350.13413081166154145Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA71200.13384882677367554No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA69490.1306341990520044Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG65740.12358457685535716No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC64860.12193026551321061Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATCCTGCTCTGCGAGGCTGGCCGTAGGCCGGCCCCGAGATCGGAAGAGC63040.11850884887377115No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC61660.11591458790540497Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA59320.1115156236546971No Hit
ACGGACGTGGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC58730.11040648309575793TruSeq Adapter, Index 5 (97% over 35bp)
AAGACTGACTAAGCATGTAGTACCGAGGAGATCGGAAGAGCACACGTCTG57890.10882736772370896Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG55080.10354485082435462No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)