FastQCFastQC Report
Fri 14 Jul 2023
SRR9691520_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691520_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5314900
Total Bases403.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA1023771.9262262695441117Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC850491.6001994393121226No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG821621.5458804493029032No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA621691.1697115655986001No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA464550.874052192891682No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG322950.6076313759431033No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC268380.5049577602588948No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT247980.4665751001900318Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG231750.43603830739995114No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG216640.4076087979077687Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA187900.35353440328134117No Hit
AGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCA179250.3372594028109654Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT174870.3290184199138272No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA162260.30529266778302505No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT159700.30047602024497166Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA146650.27592240681856667No Hit
ATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGAACTCC136700.2572014525202732Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC132580.24944966038871852No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC124670.2345669720973113No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG122430.2303524055015146No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC122260.23003255000094072Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA112920.21245931249882408Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG110470.20784963028467138No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC107650.20254379198103445No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA101500.190972548872039Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCATCATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTG100580.18924156616305104Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC98570.1854597452445013No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA97180.18284445615157385Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC90360.1700126060697285No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC89610.1686014788613144TruSeq Adapter, Index 5 (97% over 35bp)
AGAATGTGGCGCTCCGCGGTTGAGATCGGAAGAGCACACGTCTGAACTCC88670.16683286609343542Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAAGTATCTCGTATG88320.1661743400628422TruSeq Adapter, Index 3 (97% over 36bp)
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGAGATCGGAAGAGCACAC86630.16299460008654915No Hit
ATGTTGGTGGTGGTACAGGTGCAACCCTGAATATGAGATCGGAAGAGCAC84210.15844136296073302No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA82570.15535569813166758Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG80360.1511975766242074No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC79130.14888332800240833Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC78240.14720879038175697Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATAGCGTTCTGGATTGTAAACTGAGATCGGAAGAGCACACGTCTGAACTC77330.14549662270221453Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA75680.14239214284370355No Hit
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA71620.13475324088882198No Hit
ACGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC70820.1332480385331803Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA68800.12944740258518506Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG64260.12090537921691848No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC63690.11983292253852378Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATCCTGCTCTGCGAGGCTGGCCGTAGGCCGGCCCCGAGATCGGAAGAGC62010.11667199759167622No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC60500.11383092814540255Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGACGTGGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC59590.11211876046586013TruSeq Adapter, Index 5 (97% over 35bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA58870.11076407834578261No Hit
AAGACTGACTAAGCATGTAGTACCGAGGAGATCGGAAGAGCACACGTCTG57390.10797945398784549Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG54530.10259835556642646No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)