FastQCFastQC Report
Fri 14 Jul 2023
SRR9691522_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691522_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5578546
Total Bases423.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA1077201.9309691091549661Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC898851.6112621460860947No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG866871.5539353802944351No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA648141.1618439643591718No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA487540.8739553281446455No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG342800.6144970391926499No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC288050.5163531859376977No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT263000.4714490119826923Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG247110.44296488726632355No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG225600.40440645286424093Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA193880.3475457583391801No Hit
AGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCA185570.33264940362596274Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT184470.33067756365189066No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA175910.3153330634900205No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT171130.30676452251177994Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA156900.28125608357446547No Hit
ATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGAACTCC146000.26171694201320556Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC142190.25488720537573767No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC129690.2324799329431002No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC127430.22842869808727936Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG125810.22552471558000955No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA123460.22131214836267368Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG115910.2077781558133607No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC113780.20395995659083926No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA109980.19714814577131748Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCATCATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTG106830.19150151311829283Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC102630.18397266958092665No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA98190.17601360641285382Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC95160.1705820835751825No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC92760.1662798872681161TruSeq Adapter, Index 5 (97% over 35bp)
AGAATGTGGCGCTCCGCGGTTGAGATCGGAAGAGCACACGTCTGAACTCC92760.1662798872681161Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGAGATCGGAAGAGCACAC91530.16407501166074456No Hit
ATGTTGGTGGTGGTACAGGTGCAACCCTGAATATGAGATCGGAAGAGCAC90880.16290983349424742No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA85060.15247700744961143Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG84760.15193923291122813No Hit
ATAGCGTTCTGGATTGTAAACTGAGATCGGAAGAGCACACGTCTGAACTC83280.14928621185520383Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC82870.14855125331941335Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA80280.14390846647137084No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC79350.1422413654023826Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA75320.13501726077010032Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC74930.13431815387020202Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA73200.13121698736552498No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG69350.12431554745627264No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAAGTATCTCGTATG66490.11918876352368521TruSeq Adapter, Index 3 (97% over 36bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC65910.11814906608281082Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATCCTGCTCTGCGAGGCTGGCCGTAGGCCGGCCCCGAGATCGGAAGAGC63920.11458182831153493No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC63270.1134166501450378Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGACGTGGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC62870.11269961742719341TruSeq Adapter, Index 5 (97% over 35bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA62020.11117592290177404No Hit
AAGACTGACTAAGCATGTAGTACCGAGGAGATCGGAAGAGCACACGTCTG58930.10563684515642606Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG58610.10506321898215054No Hit
TCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAAC55900.10020532231875473Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)