FastQCFastQC Report
Fri 14 Jul 2023
SRR9691523_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691523_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638017
Total Bases124.4 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA304231.8573067312488212No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC260141.5881398056308327No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG236161.4417432786106614No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG152260.9295385823224056No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC146910.8968771386377553No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA102410.6252071864944014Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA91960.5614105348112993No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT90460.5522531206941076No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG87370.5333888476126927No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA73140.44651551235426734No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA54360.3318646876070273No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT51910.3169075778822808No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG50800.310131091435559No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA50150.30616287865144254Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCGCTGGGTCGTCGTTTTCGTGCCCTGAAACTGTGGTTAGATCGGAAG43730.26696914622986206No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAACATCTCGTATG42520.25958216550866076TruSeq Adapter, Index 22 (97% over 37bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT42140.2572622872656389No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGCAGATCGGAAGAGCACACGTCTG41710.2546371618853772Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA41090.2508520973836047No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAACATC38060.23235412086687746TruSeq Adapter, Index 22 (97% over 37bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC37610.22960689663171996Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA36720.22417349758885288No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGAGATCGGAAGAGCACACGTC35600.21733596171468308No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA35400.21611497316572414Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA34170.20860589358962697No Hit
AAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT33060.2018294071429051Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC32800.20024212202925853No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA29350.17918006955971763No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28750.17551710391284095No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG28270.17258673139533962No Hit
TCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACGTCTGA27330.1668480852152328Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA25220.15396665602371648Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC24590.15012054209449596Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC24170.1475564661416823No Hit
AAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCCA23620.14419874763204535Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG22530.13754436004021936No Hit
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAACAT22110.13498028408740567TruSeq Adapter, Index 22 (97% over 37bp)
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA21570.13168361500521666No Hit
TGTTCCGCTGGGTCGTCGTTTTCGTGCCCTGAAACTGTGGTTAGATCGGA21510.131317318440529No Hit
TTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACGTCTGAAC20110.12277039859781676Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG19560.11941268008817979No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA19550.11935163066073184Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC19300.11782539497453323Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC19220.11733699955494967No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGAGATCGGAAGAGCACAC18500.11294144077869765No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC18200.11110995795525931No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG18170.11092680967291549No Hit
ATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCACA18080.11037736482588398No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC17780.10854588200244564Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG17580.10732489345348674No Hit
AAACTGCGACGACACATTCACCAGATCGGAAGAGCACACGTCTGAACTCC17530.10701964631624702Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA16670.10176939555572379Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG16600.10134204956358817No Hit
TCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACGTCTGAACT16590.10128100013614022Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)