FastQCFastQC Report
Fri 14 Jul 2023
SRR9691525_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691525_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706217
Total Bases129.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA314531.8434349206460845No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC269501.5795177284014872No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG242261.4198662889890326No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG156350.9163547192414565No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC150340.881130594760221No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA104730.613814069371012Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT97870.5736081635571559No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA95690.5608313596687877No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG91710.5375049011936934No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA75070.4399792054586257No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA57120.33477570555210734No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT53630.3143210974922885No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA52010.304826408364235Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG51780.30347839694482004No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAACATCTCGTATG45210.2649721577032699TruSeq Adapter, Index 22 (97% over 37bp)
TTCCGCTGGGTCGTCGTTTTCGTGCCCTGAAACTGTGGTTAGATCGGAAG44720.26210030728799444No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT43960.25764600868471005No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGCAGATCGGAAGAGCACACGTCTG43140.25284005492853484Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA42070.24656887136864772No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA40690.23848080285215775No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAACATC40120.23514007889969446TruSeq Adapter, Index 22 (97% over 37bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGAGATCGGAAGAGCACACGTC36980.2167367925650723No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC36910.21632652822003295Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA35980.2108758733502245Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA35600.2086487240485823No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC34720.20349111513951623No Hit
AAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT34330.20120535664572559Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA30350.1778788981706313No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG29070.17037692157562606No Hit
TCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACGTCTGA26580.155783232730655Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA26490.15525575000131872Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC24650.14447165864599873No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC24390.1429478196501383Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCCA23820.13960709569767504Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTTCCGCTGGGTCGTCGTTTTCGTGCCCTGAAACTGTGGTTAGATCGGA23520.1378488199332207No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG23300.13655941770595417No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA23040.13503557871009372No Hit
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAACAT22880.13409783163571806TruSeq Adapter, Index 22 (97% over 37bp)
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA22730.1332186937534909No Hit
TTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACGTCTGAAC21230.1244273149312192Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA20230.11856639571637137Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGAGATCGGAAGAGCACAC20110.11786308541058962No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC19920.11674951075976855Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC19680.11534289014820506No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG19590.11481540741886875No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC19560.11463957984242333No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG19470.11411209711308702No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC19400.11370183276804768Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCACA18710.10965779850980269No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG18320.10737204001601203No Hit
AAACTGCGACGACACATTCACCAGATCGGAAGAGCACACGTCTGAACTCC18280.10713760324741811Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACGTCTGAACT17740.10397270687140028Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG17290.10133529322471878No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)