FastQCFastQC Report
Fri 14 Jul 2023
SRR9691526_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691526_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676995
Total Bases127.4 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA314081.872873801054863No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC265091.5807441286348498No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG241631.4408510460675195No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG157890.9415054904755231No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC150800.899227487261441No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA106720.6363763756004043Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT98020.5844978667199365No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA96280.574122164943843No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG91530.5457976917045072No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA74820.4461551763720226No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA57490.34281557190093No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT53990.32194490740878773No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA52300.31186735798258197Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG50980.3039961359455454No Hit
TTCCGCTGGGTCGTCGTTTTCGTGCCCTGAAACTGTGGTTAGATCGGAAG46060.2746579447165913No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAACATCTCGTATG45110.26899305006872415TruSeq Adapter, Index 22 (97% over 37bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT44430.2649381781102508No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA43230.25778252171294486No Hit
TTCTGCGTCGCGAAACTCTGGAAGATGCAGATCGGAAGAGCACACGTCTG42960.25617249902355105Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAACATC39390.23488442124156603TruSeq Adapter, Index 22 (97% over 37bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC38960.23232031103253142Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA36990.22057310844695424No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA36970.22045384750699915Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGAGATCGGAAGAGCACACGTC35600.2122844731200749No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35430.21127075513045654No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC34880.20799107928169136No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA34330.20471140343292618No Hit
AAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT33930.20232618463382418Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA30340.18091884591188406No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG28210.16821755580666609No Hit
TCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACGTCTGA27280.162671922098754Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA26980.16088300799942754Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC25130.14985137105358096No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC24350.1452001943953321Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA23790.1418608880765894No Hit
AAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCCA23270.13876010363775684Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTTCCGCTGGGTCGTCGTTTTCGTGCCCTGAAACTGTGGTTAGATCGGA22340.13321446992984473No Hit
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAACAT22210.13243927382013662TruSeq Adapter, Index 22 (97% over 37bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG22060.13154481677047336No Hit
TTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACGTCTGAAC21550.12850366280161837Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA20110.1199168751248513Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG19910.11872426572530032No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC19880.11854537431536766No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG19770.11788943914561463No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC19770.11788943914561463Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC19410.11574274222642285Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGAGATCGGAAGAGCACAC19200.11449050235689433No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC19070.11371530624718618No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG18830.112284174967725No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA18600.11091267415824138No Hit
ATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCACA18580.11079341321828629No Hit
AAACTGCGACGACACATTCACCAGATCGGAAGAGCACACGTCTGAACTCC18440.10995858663860059Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG17610.10500925763046401No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG17280.10304145212120489No Hit
TCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACA17230.10274329977131715No Hit
TCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACGTCTGAACT17000.10137179896183351Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)