FastQCFastQC Report
Fri 14 Jul 2023
SRR9691527_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691527_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020453
Total Bases153.5 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA701903.4739734109132954No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG471832.3352683779330676No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC306931.5191147727762042No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG176790.8750017941521036No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA143720.7113256284605481Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA132140.6540117488503815No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA92410.45737267830531075No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC90740.44910720516636615No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA81440.4030779236141597Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA65540.32438270031522637No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA64800.3207201553315024No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT62510.3093860634224107No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA57990.2870148427110158No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC57800.2860744595395191Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG56850.2813725436820357No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATC56340.278848357274334TruSeq Adapter, Index 22 (97% over 42bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG54820.27132529190236054No Hit
TTCCGCTGGGTCGTCGTTTTCGTGCCCTGAAACTGTGGTTAGATCGGAAG53300.263802226530387No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC52570.26018917539779446Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA46620.23074033397460866Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA43360.21460533850577074No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG42920.21242760905598895No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC37270.1844635831667453No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA35470.17555468996309245Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG34330.1699123909341123No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG33960.16808111844225032TruSeq Adapter, Index 22 (97% over 49bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT33750.16704174756849083No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA33380.16521047507662884No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA32780.16224084400874458Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT32050.15862779287615203No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG30900.1529359999960405No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC30150.14922396116118514No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT30050.1487290226498711No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC30010.14853104724534547Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG29490.14595736698651243No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA29430.145660403879724Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTTCCGCTGGGTCGTCGTTTTCGTGCCCTGAAACTGTGGTTAGATCGGA26140.12937692685749186No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26090.12912945760183484No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG25880.12809008672807534TruSeq Adapter, Index 2 (100% over 35bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT25810.12774362977015552Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCCA25630.12685274044979022Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG25520.12630830808734475No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG25350.12546691261811088No Hit
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGAT23880.11819131650179439TruSeq Adapter, Index 22 (97% over 41bp)
ATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCACA23810.11784485954387458No Hit
AAACTGCGACGACACATTCACCAGATCGGAAGAGCACACGTCTGAACTCC23450.11606308090314399Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT23350.11556814239182996Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC21970.10873799093569611No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGAGC21880.10829254627551345No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA21830.10804507701985644No Hit
AAGACTGACTAAGCATGTAGTACCGAGGAGATCGGAAGAGCACACGTCTG21600.10690671844383412Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGA21520.10651076763478289No Hit
AGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGAGCA21420.10601582912346885No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)