FastQCFastQC Report
Fri 14 Jul 2023
SRR9691528_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691528_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2122341
Total Bases161.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA733173.4545344032839207No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG492872.322294108251219No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC321191.5133760314671392No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG189180.8913741948160074No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA152450.7183105825124237Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA139270.6562093461889489No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA102410.48253320272284234No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC95640.4506344644899194No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA86870.4093121699104903Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA73010.3440069244292034No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA67580.3184219689484395No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT63640.29985756294582255No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATC62920.2964650826610804TruSeq Adapter, Index 22 (97% over 42bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA60190.2836019282480996No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG58910.27757085218633576No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC58220.27431972524679116Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC57450.27069165605338635Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG56490.26616834900706343No Hit
TTCCGCTGGGTCGTCGTTTTCGTGCCCTGAAACTGTGGTTAGATCGGAAG53730.2531638412488851No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA50340.2371909132415573Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA46670.2198986873457187No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG45580.2147628491368729No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC38010.1790946883653475No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG37180.1751839124815475TruSeq Adapter, Index 22 (97% over 49bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA37130.17494832357288487Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA35740.16839895191206314No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG33640.15850421774823178No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC33120.15605409309814022Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA32900.15501750190002453Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT32350.1524260239047354No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG32200.15171925717874743No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT32050.15101249045275947No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT31910.15035284150850406No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG31580.14879795471133056No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA31030.1462064767160414Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC30060.14163605188798598No Hit
AAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCCA29340.13824357160324377Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT27710.13056337318084135Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG27670.13037490205391122TruSeq Adapter, Index 2 (100% over 35bp)
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG27300.12863154412980762No Hit
TGTTCCGCTGGGTCGTCGTTTTCGTGCCCTGAAACTGTGGTTAGATCGGA26880.12665259729704134No Hit
AAACTGCGACGACACATTCACCAGATCGGAAGAGCACACGTCTGAACTCC26610.12538041719026302Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG26590.12528618162679794No Hit
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGAT25610.12066863901701TruSeq Adapter, Index 22 (97% over 41bp)
AAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT25260.11901951665637142Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCACA24770.11671074535147745No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC23390.11020849147238827No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGAGC23380.11016137369065575No Hit
AGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGAGCA22830.10756989569536658No Hit
AAGACTGACTAAGCATGTAGTACCGAGGAGATCGGAAGAGCACACGTCTG22760.10724007122323885Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGA22590.10643906893378587No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA22300.10507265326354248No Hit
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA21610.1018215263239979No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAAGATCGGAA21550.1015388196336027No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)