FastQCFastQC Report
Fri 14 Jul 2023
SRR9691529_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691529_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2131081
Total Bases161.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA733043.4397566305551033No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG495152.3234686996880924No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC320241.5027115346624553No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG192780.904611321671959No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA152180.7140976809422073Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA139690.6554889279196803No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA103770.48693597287010676No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC97900.4593912666857806No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA86270.4048180242796966Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA70520.3309118705483274No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA67420.3163652625123118No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT62620.2938414823275136No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA61900.29046291529979384No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATC61470.28844515999157233TruSeq Adapter, Index 22 (97% over 42bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG60520.28398732849666436No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG59050.27708942081506993No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC59020.27694864718891493Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC57060.267751436946789Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCGCTGGGTCGTCGTTTTCGTGCCCTGAAACTGTGGTTAGATCGGAAG54170.2541902442938584No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA49790.23363729487523Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA47450.22265695203514085No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG45260.21238047732582666No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC40150.1884020363374269No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA36760.17249461658191312Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG36720.1723069184137065TruSeq Adapter, Index 22 (97% over 49bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA35020.16432974626492378No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA34610.16240584004080558Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG34530.16203044370439226No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT33120.1554140832751078No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT33090.1552733096489528No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG33070.1551794605648495No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT32620.15306785617252466No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC32270.15142549720071644Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG30820.14462143860322532No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC30730.14419911772476035No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA30640.14377679684629538Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACTGCGACGACACATTCACAGATCGGAAGAGCACACGTCTGAACTCCA28140.13204566133337964Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG27710.13002790602515812No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT27640.1296994342307965Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG27220.12772860346462664TruSeq Adapter, Index 2 (100% over 35bp)
TGTTCCGCTGGGTCGTCGTTTTCGTGCCCTGAAACTGTGGTTAGATCGGA26870.12608624449281844No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG26550.12458465914716521No Hit
AAACTGCGACGACACATTCACCAGATCGGAAGAGCACACGTCTGAACTCC25840.12125301666149714Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT25230.1183906195963457Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCACA24850.1166074869983825No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC24660.11571592069940093No Hit
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGAT24110.11313507088655944TruSeq Adapter, Index 22 (97% over 41bp)
AGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGAGCA23790.11163348554090624No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGAGC23470.11013190019525303No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA22100.10370323793417519No Hit
AAGACTGACTAAGCATGTAGTACCGAGGAGATCGGAAGAGCACACGTCTG22050.10346861522391687Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGA21740.10201395442031533No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)