FastQCFastQC Report
Fri 14 Jul 2023
SRR9691531_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691531_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2805589
Total Bases213.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC589512.101198714423246Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGGCTGAGGCGTGATGACGAGAGATCGGAAGAGCACACGTCTGAACTCC521991.8605362367759497Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC232420.8284178473753641Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTCC169120.6027967745810238Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG155150.5530033087526363No Hit
AGGCTGAGGCGTGATGACGAGAGATCGGAAGAGCACACGTCTGAACTCCA151210.5389599118046157Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAGATCGGAAGA150360.5359302449503474No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC144290.5142948592969248Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA129590.4618994442878126No Hit
AAGGCTGAGGCGTGATGACGAGGCAGATCGGAAGAGCACACGTCTGAACT117520.4188781749572015Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGCTGAGGCGTGATGACGAGATCGGAAGAGCACACGTCTGAACTCCAG112100.39955959336880775Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTGGACCGTTCGTAGCATTCATGAGATCGGAAGAGCACACGTCTGAACT111110.3960309225620716Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA103290.36815798750280243Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA100110.35682346915389246Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCATGGCCTTTACCGAACATAGTCCAGATCGGAAGAGCACACGTCTGA98530.35119185311889944Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC97120.3461661704547601Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGCATCGGTTCTGACGCTTACAACCAGATCGGAAGAGCACACGTCTGA94400.3364712365211013Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG86440.3080992975093643No Hit
AGCCAGTGGACCGTTCGTAGCATTCATGATCTGCGAGATCGGAAGAGCAC84320.30054295194342434No Hit
AGTGGACCGTTCGTAGCATTCATGATCTGCGAGATCGGAAGAGCACACGT83210.29658656346314444No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC76240.27174329525814367No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC73830.26315329864780623No Hit
TCCATGGCCTTTACCGAACATAGTCCAGATCGGAAGAGCACACGTCTGAA71480.2547771608742407Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC70310.2506069135571889No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC68230.2431931405490968No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT67950.24219513264416137Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA65420.23317741836028014Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA56660.20195402819158473No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCAGATCGGAAGAGCACACGT54780.1952531179727323No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA53780.19168880402653415No Hit
TCCTCTGCAGTTCAGTCGGTAGAACAGATCGGAAGAGCACACGTCTGAAC52950.18873042345118976Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGACCGCACTGACCGAAAGCTATGAGATCGGAAGAGCACACGTCTGAACT52950.18873042345118976Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT52840.18833834891710796Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTGACTCTGTGACTCTGGTGCAGATCGGAAGAGCACACGTCTGAACT51350.18302752113727278Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAAGAGC49130.175114744176713No Hit
AGCCAGTGGACCGTTCGTAGCAGATCGGAAGAGCACACGTCTGAACTCCA49050.17482959906101714Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAAGGAGATCAACAATGAAAGCAGATCGGAAGAGCACACGTCTGAACT48780.17386723429554365Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTAGAGGGTATTAATAATGAAAGCTACTAAACAGATCGGAAGAGCACA48210.17183557534621074No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC47670.16991084581526375No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGAGATCGGAAGAGCA46390.16534852396413016No Hit
ATCAGGGTCTGAACAAAAACATCAGATCGGAAGAGCACACGTCTGAACTC45800.16324557873587328Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG43380.15461993898607385No Hit
AGCTGTCTTCTGACGTTCAGACTCAGATCGGAAGAGCACACGTCTGAACT43350.15451300956768793Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC42860.15276649573405085No Hit
ATCTGAGCAGCGATATTATTGGTCAGATCGGAAGAGCACACGTCTGAACT42830.1526595663156649Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42680.1521249192237352No Hit
TTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTC42260.150627907366332Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCAGATCGGAAGAGCACA41360.1474200248147537No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA36860.13138061205686222No Hit
ACGATCGTGACTCTGTGACTCTGGAGATCGGAAGAGCACACGTCTGAACT36840.13130932577793825Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG35950.12813708636582194No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG33490.11936887405817459No Hit
ATCTGACCGCACTGACCGAAAGCAGATCGGAAGAGCACACGTCTGAACTC32270.1150204110438129Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG31550.11245410500255026No Hit
TGACCGTGCAGACCATTGATAATGAGATCGGAAGAGCACACGTCTGAACT31040.11063630488998924Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGA31030.11060066175052725No Hit
AAAAGGAGATCAACAATGAAAGCAGATCGGAAGAGCACACGTCTGAACTC30800.1097808695429017Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGGGTCTGAACAAAAACATCAGATCGGAAGAGCACACGTCTGAACTCC29810.10625219873616557Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAA29470.1050403319944582No Hit
AGCTGACCAGACAGTCGCCGCTTCGTCGTCGTCCTCAGATCGGAAGAGCA29310.1044700417630665No Hit
TGGACCGTTCGTAGCATTCATGATCTGCGAGATCGGAAGAGCACACGTCT29150.10389975153167481Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCTGCAGTTCAGTCGGTAGAACGAGATCGGAAGAGCACACGTCTGAA29030.10347203385813104Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAGATCGGAAGAGCACACG28670.10218888083749972No Hit
AATCTAGAGGGTATTAATAATGAAAGCTACTAAACAGATCGGAAGAGCAC28210.1005492964222486No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)