FastQCFastQC Report
Fri 14 Jul 2023
SRR9691532_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691532_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2786119
Total Bases211.7 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC594382.133361855685274Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGGCTGAGGCGTGATGACGAGAGATCGGAAGAGCACACGTCTGAACTCC519691.8652828540345907Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC232570.8347453931436525Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTCC164830.5916114853672797Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG158460.5687481403342787No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAGATCGGAAGA152390.5469615619433341No Hit
AGGCTGAGGCGTGATGACGAGAGATCGGAAGAGCACACGTCTGAACTCCA149120.5352248055449175Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC141360.5073724417370543Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA130860.46968560926507447No Hit
AAGGCTGAGGCGTGATGACGAGGCAGATCGGAAGAGCACACGTCTGAACT117910.42320518254963263Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTGGACCGTTCGTAGCATTCATGAGATCGGAAGAGCACACGTCTGAACT111180.39904971754616364Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGCTGAGGCGTGATGACGAGATCGGAAGAGCACACGTCTGAACTCCAG110540.39675261537644296Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA103330.3708743237456835Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA100340.3601425495465197Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC96370.34589333764997116Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCCATGGCCTTTACCGAACATAGTCCAGATCGGAAGAGCACACGTCTGA95100.3413350255319317Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCATCGGTTCTGACGCTTACAACCAGATCGGAAGAGCACACGTCTGA93430.3353410245578168Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCAGTGGACCGTTCGTAGCATTCATGATCTGCGAGATCGGAAGAGCAC85950.30849364294920645No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG85230.30590940300827063No Hit
AGTGGACCGTTCGTAGCATTCATGATCTGCGAGATCGGAAGAGCACACGT80870.29026039447704854No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC76020.272852667097134No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC73880.26517173171713054No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC72140.25892648519320244No Hit
TCCATGGCCTTTACCGAACATAGTCCAGATCGGAAGAGCACACGTCTGAA71650.25716776634451005Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC70090.25156857980581593No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT67690.2429544466693634Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA63130.22658759371010354Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA56620.20322175757747607No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCAGATCGGAAGAGCACACGT55370.19873522990224035No Hit
TCCTCTGCAGTTCAGTCGGTAGAACAGATCGGAAGAGCACACGTCTGAAC54160.1943922711126122Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT53310.19134143229345194Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA53300.19130554007205006No Hit
TGACCGCACTGACCGAAAGCTATGAGATCGGAAGAGCACACGTCTGAACT52420.18814702458868413Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAAGAGC50460.18111214919391455No Hit
ATCGTGACTCTGTGACTCTGGTGCAGATCGGAAGAGCACACGTCTGAACT50260.18039430476587684Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCAGTGGACCGTTCGTAGCAGATCGGAAGAGCACACGTCTGAACTCCA49410.17734346594671657Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAAGGAGATCAACAATGAAAGCAGATCGGAAGAGCACACGTCTGAACT49190.17655383707587508Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48900.1755129626552204No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC48440.17386192047073368No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGAGATCGGAAGAGCA45710.16406334402801892No Hit
ATCTAGAGGGTATTAATAATGAAAGCTACTAAACAGATCGGAAGAGCACA45280.16251997850773783No Hit
ATCAGGGTCTGAACAAAAACATCAGATCGGAAGAGCACACGTCTGAACTC44890.1611201818730643Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG42810.1536545998214721No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC42020.15081911433072315No Hit
AGCTGTCTTCTGACGTTCAGACTCAGATCGGAAGAGCACACGTCTGAACT41820.1501012699026854Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTC41720.14974234768866657Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCAGATCGGAAGAGCACA41630.1494193176960496No Hit
ATCTGAGCAGCGATATTATTGGTCAGATCGGAAGAGCACACGTCTGAACT41270.14812719772558172Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG37660.13517010579950103No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA37630.13506242913529537No Hit
ACGATCGTGACTCTGTGACTCTGGAGATCGGAAGAGCACACGTCTGAACT37120.13323192584379923Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG32700.11736756398416578No Hit
ATCTGACCGCACTGACCGAAAGCAGATCGGAAGAGCACACGTCTGAACTC32330.11603955179229603Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAAGGAGATCAACAATGAAAGCAGATCGGAAGAGCACACGTCTGAACTC31540.11320406630154706Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG31090.11158891633846221No Hit
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGA30780.11047625747500375No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAA30400.10911235306173211No Hit
TGGACCGTTCGTAGCATTCATGATCTGCGAGATCGGAAGAGCACACGTCT29870.10721006532743217Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGACCGTGCAGACCATTGATAATGAGATCGGAAGAGCACACGTCTGAACT29800.10695881977761898Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTGACCAGACAGTCGCCGCTTCGTCGTCGTCCTCAGATCGGAAGAGCA29620.10631275979238503No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGAGATCGGAAGAGCACACGTCTGAA29280.10509242426472093Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCAGGGTCTGAACAAAAACATCAGATCGGAAGAGCACACGTCTGAACTCC29170.10469760982930018Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGGCTGAGGCGTGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA28130.10096481880350408Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AATCTAGAGGGTATTAATAATGAAAGCTACTAAACAGATCGGAAGAGCAC27950.10031875881827015No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)