FastQCFastQC Report
Fri 14 Jul 2023
SRR9691535_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691535_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3759164
Total Bases285.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC566951.5081810743026907Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC259770.6910313037686039Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA258270.6870410548728387No Hit
AAGGCTGAGGCGTGATGACGAGAGATCGGAAGAGCACACGTCTGAACTCC218800.5820443055956058Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC213460.567839019526682Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA208140.5536869367763684No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG198190.5272182857677931No Hit
ACCGCATCGGTTCTGACGCTTACAACCAGATCGGAAGAGCACACGTCTGA169990.45220160652740876Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCATGGCCTTTACCGAACATAGTCCAGATCGGAAGAGCACACGTCTGA149840.3985992630276306Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC128840.3427357784869189No Hit
AGCCAGTGGACCGTTCGTAGCATTCATGATCTGCGAGATCGGAAGAGCAC126720.33709622671423756No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA121700.3237421937430769Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGGACCGTTCGTAGCATTCATGAGATCGGAAGAGCACACGTCTGAACT101590.2702462568805192Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTGGACCGTTCGTAGCATTCATGATCTGCGAGATCGGAAGAGCACACGT97030.2581159002373932No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT95320.25356701649622093Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTCC92680.24654417843967433Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCATGGCCTTTACCGAACATAGTCCAGATCGGAAGAGCACACGTCTGAA91950.244602257310402Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTGTCTTCTGACGTTCAGACTCAGATCGGAAGAGCACACGTCTGAACT86240.22941270984718942Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC80570.21432956902119726No Hit
AAGGCTGAGGCGTGATGACGAGGCAGATCGGAAGAGCACACGTCTGAACT77600.2064288762075823Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC76230.2027844488827835No Hit
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG76130.2025184322897325Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTGACTCTGTGACTCTGGTGCAGATCGGAAGAGCACACGTCTGAACT75120.19983166469991734Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG74640.19855478505327248No Hit
ATCAGGGTCTGAACAAAAACATCAGATCGGAAGAGCACACGTCTGAACTC69980.18615841181709553Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC66530.17698083935683573Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA63230.16820229178615245Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCTAGAGGGTATTAATAATGAAAGCTACTAAACAGATCGGAAGAGCACA61660.16402583127525164No Hit
AGCCAGTGGACCGTTCGTAGCAGATCGGAAGAGCACACGTCTGAACTCCA60140.15998237906087628Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG59270.15766803470133253No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC54780.14572388967334227No Hit
AAGGCTGAGGCGTGATGACGAGATCGGAAGAGCACACGTCTGAACTCCAG52910.1407493793832884Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA47520.12641108501783907Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTGAGCAGCGATATTATTGGTCAGATCGGAAGAGCACACGTCTGAACT46590.1239371307024647Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC46280.12311247926400656TruSeq Adapter, Index 5 (100% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATCGATCTCGTATG46050.12250064109998926TruSeq Adapter, Index 8 (97% over 37bp)
AATCTAGAGGGTATTAATAATGAAAGCTACTAAACAGATCGGAAGAGCAC45000.11970746687295367No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC44620.11869660381935983No Hit
TTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTC44020.11710050426105378Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA43180.11486596487942531No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC42440.11289744209084787No Hit
TGGACCGTTCGTAGCATTCATGATCTGCGAGATCGGAAGAGCACACGTCT41930.11154075746628771Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAGGGTCTGAACAAAAACATCAGATCGGAAGAGCACACGTCTGAACTCC41440.11023727616033777Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCGTTCACCTCTGAAGAATTCACTCAGATCGGAAGAGCACACGTCTGA40700.10826875337176033Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACAGTGGGCTACTGGTCTGAAGAGATCGGAAGAGCACACGTCTGAACTC39450.10494354595862272Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGGAAGAGCACACG39370.10473073268418191No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC39310.10457112272835131No Hit
ATCTGACCGCACTGACCGAAAGCTATGAGATCGGAAGAGCACACGTCTGA38630.10276220989560444Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTGACTCTGTGACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTC37730.10036806055814537Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGACCGTGCAGACCATTGATAATGAGATCGGAAGAGCACACGTCTGAACT37640.10012864562439948Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)