FastQCFastQC Report
Fri 14 Jul 2023
SRR9691536_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691536_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3574066
Total Bases271.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC552561.546026290504988Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC249330.6976088298313462Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA247600.692768404388727No Hit
AAGGCTGAGGCGTGATGACGAGAGATCGGAAGAGCACACGTCTGAACTCC207660.5810189291412078Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC202860.567588846988276Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA198560.5555577317262748No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG191580.5360281539288866No Hit
ACCGCATCGGTTCTGACGCTTACAACCAGATCGGAAGAGCACACGTCTGA164080.45908497492771533Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCATGGCCTTTACCGAACATAGTCCAGATCGGAAGAGCACACGTCTGA146060.40866620817858423Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC126300.35337903664901543No Hit
AGCCAGTGGACCGTTCGTAGCATTCATGATCTGCGAGATCGGAAGAGCAC119110.3332618927574365No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA118160.3306038556646687Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGGACCGTTCGTAGCATTCATGAGATCGGAAGAGCACACGTCTGAACT98690.27612808493183955Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTGGACCGTTCGTAGCATTCATGATCTGCGAGATCGGAAGAGCACACGT94030.26308971350836835No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT89570.2506109288412693Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTCC88950.24887620989651563Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCATGGCCTTTACCGAACATAGTCCAGATCGGAAGAGCACACGTCTGAA85860.24023059451056583Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTGTCTTCTGACGTTCAGACTCAGATCGGAAGAGCACACGTCTGAACT83390.23331969806936972Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC77920.21801500028259133No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC76000.2126429674214186No Hit
AAGGCTGAGGCGTGATGACGAGGCAGATCGGAAGAGCACACGTCTGAACT74320.20794243866789253Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG73830.20657145111478073Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTGACTCTGTGACTCTGGTGCAGATCGGAAGAGCACACGTCTGAACT70860.1982615877826543Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG70140.1962470754597145No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67020.1875175220603089No Hit
ATCAGGGTCTGAACAAAAACATCAGATCGGAAGAGCACACGTCTGAACTC65850.18424393953553178Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC62920.17604599355467973Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA62500.17487086136629823Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCTAGAGGGTATTAATAATGAAAGCTACTAAACAGATCGGAAGAGCACA58790.16449052703559475No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG58490.16365114690103652No Hit
AGCCAGTGGACCGTTCGTAGCAGATCGGAAGAGCACACGTCTGAACTCCA57030.1595661635795198Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC53310.14915784991099773No Hit
AAGGCTGAGGCGTGATGACGAGATCGGAAGAGCACACGTCTGAACTCCAG50440.14112777995705733Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA45930.12850909860086523Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC45120.126242772237558TruSeq Adapter, Index 5 (100% over 35bp)
ATCTGAGCAGCGATATTATTGGTCAGATCGGAAGAGCACACGTCTGAACT44530.12459199130626016Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATCGATCTCGTATG44310.12397644587425079TruSeq Adapter, Index 8 (97% over 37bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC41690.11664585936577555No Hit
AATCTAGAGGGTATTAATAATGAAAGCTACTAAACAGATCGGAAGAGCAC41670.11658990069013835No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC41610.1164220246632267No Hit
TTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTC41450.11597435525812898Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGACCGTTCGTAGCATTCATGATCTGCGAGATCGGAAGAGCACACGTCT41070.11491114042102189Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAGGGTCTGAACAAAAACATCAGATCGGAAGAGCACACGTCTGAACTCC41050.11485518174538466Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA40650.11373600823264035No Hit
TGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGGAAGAGCACACG39520.1105743430591377No Hit
ATCGTGACTCTGTGACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTC38690.10825205802019325Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACAGTGGGCTACTGGTCTGAAGAGATCGGAAGAGCACACGTCTGAACTC38240.1069929878183559Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGCGTTCACCTCTGAAGAATTCACTCAGATCGGAAGAGCACACGTCTGA37870.10595775231906741Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTGACCGCACTGACCGAAAGCTATGAGATCGGAAGAGCACACGTCTGA35960.10061369879571333Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC35940.10055774012007614No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)