FastQCFastQC Report
Fri 14 Jul 2023
SRR9691537_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691537_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3744294
Total Bases284.5 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC564981.5089092897085539Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC258920.6915055281449587Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA255380.6820511423515354No Hit
AAGGCTGAGGCGTGATGACGAGAGATCGGAAGAGCACACGTCTGAACTCC214790.5736461933811822Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC209570.559704980431558Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA206440.5513455941226837No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG193940.5179614634961891No Hit
ACCGCATCGGTTCTGACGCTTACAACCAGATCGGAAGAGCACACGTCTGA170530.45543966365889Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCATGGCCTTTACCGAACATAGTCCAGATCGGAAGAGCACACGTCTGA150460.4018381035249903Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC126650.3382480115076434No Hit
AGCCAGTGGACCGTTCGTAGCATTCATGATCTGCGAGATCGGAAGAGCAC123470.32975508867626313No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA122140.32620301717760414Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGGACCGTTCGTAGCATTCATGAGATCGGAAGAGCACACGTCTGAACT102590.2739902368777665Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTGGACCGTTCGTAGCATTCATGATCTGCGAGATCGGAAGAGCACACGT94310.2518765887507765No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT93160.24880524873313903Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTCC92500.24704256663606008Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCATGGCCTTTACCGAACATAGTCCAGATCGGAAGAGCACACGTCTGAA88110.2353180599600352Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTGTCTTCTGACGTTCAGACTCAGATCGGAAGAGCACACGTCTGAACT86020.2297362333192853Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC80470.2149136793211217No Hit
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG77300.20644746379424264Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGCTGAGGCGTGATGACGAGGCAGATCGGAAGAGCACACGTCTGAACT77280.2063940491852403Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC75760.2023345389010585No Hit
ATCGTGACTCTGTGACTCTGGTGCAGATCGGAAGAGCACACGTCTGAACT74670.19942344271042817Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG74310.19846197974838514No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC69050.18441393758075622Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCAGGGTCTGAACAAAAACATCAGATCGGAAGAGCACACGTCTGAACTC68970.18420027914474665Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA63000.16825601835753282Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCTAGAGGGTATTAATAATGAAAGCTACTAAACAGATCGGAAGAGCACA61770.16497101990388574No Hit
AGCCAGTGGACCGTTCGTAGCAGATCGGAAGAGCACACGTCTGAACTCCA59800.1597096809171502Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG57200.15276578174683933No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC54940.1467299309295691No Hit
AAGGCTGAGGCGTGATGACGAGATCGGAAGAGCACACGTCTGAACTCCAG52020.13893139801521995Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA47360.12648579411766275Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTGAGCAGCGATATTATTGGTCAGATCGGAAGAGCACACGTCTGAACT47180.12600506263664124Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATCGATCTCGTATG46420.12397530749455037TruSeq Adapter, Index 8 (97% over 37bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC45830.12239957652897981TruSeq Adapter, Index 5 (100% over 35bp)
AATCTAGAGGGTATTAATAATGAAAGCTACTAAACAGATCGGAAGAGCAC44790.11962201686085548No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC42900.1145743363101295No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC42850.1144407997876235No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA42750.11417372674261156No Hit
TTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTC42720.11409360482910796Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGACCGTTCGTAGCATTCATGATCTGCGAGATCGGAAGAGCACACGTCT41910.11193031316451112Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAGGGTCTGAACAAAAACATCAGATCGGAAGAGCACACGTCTGAACTCC41900.11190360586000993Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGGAAGAGCACACG39390.1052000724302098No Hit
AAGCGTTCACCTCTGAAGAATTCACTCAGATCGGAAGAGCACACGTCTGA39310.10498641399420024Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACAGTGGGCTACTGGTCTGAAGAGATCGGAAGAGCACACGTCTGAACTC39090.10439885329517394Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTGACTCTGTGACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTC38740.10346409763763208Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGACCGTGCAGACCATTGATAATGAGATCGGAAGAGCACACGTCTGAACT38050.10162129362704958Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC37660.10057970875150296No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG37620.10047287953349818No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)