FastQCFastQC Report
Fri 14 Jul 2023
SRR9691541_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691541_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5477811
Total Bases416.3 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG820261.4974229669479286No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC807331.4738186476313258No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA648651.1841408913158924Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA625181.141295309385446No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC439920.8030945207857665Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG364000.6644990124704924No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC360030.6572515919223938No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA323210.590034961045571No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT309600.5651892699474298Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT251520.4591615154301599No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG208800.3811741588017549No Hit
AGTCCGGATCGTCCGAGCTTTGATAAAGCAGATCGGAAGAGCACACGTCT199940.3649998147070061Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG184070.3360283879819877Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGCCATCTCGTATG179850.3283245807495001TruSeq Adapter, Index 20 (97% over 39bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA177710.32441791073112963No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA163960.29931664308973055No Hit
AGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCA162020.29577508241887135Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG147570.26939593206118284Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT136820.24977130463245262TruSeq Adapter, Index 18 (100% over 35bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG135920.24812831256865198No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC132610.2420857528673406No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA131310.23971254210851745Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT128480.2345462448412331Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA125980.2299823779973424Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA123770.22594791970734296Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT123020.22457875965417573Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA119920.2189195647677512No Hit
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT115480.21081413725300124Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA114920.2097918310799697Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA112130.20469855568218767No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA108100.19734160232983575No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC99940.18244514095137637No Hit
AGTCCGGATCGTCCGAGCTTTGATAAAGCAAGATCGGAAGAGCACACGTC94450.17242288936219233No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA91380.16681846087789445No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG90910.165960453911243No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA85200.15553658203979653Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGGATCGTCCGAGCTTTGATAAAGCATTTCAGAGATCGGAAGAGCA84940.15506193988803194No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC84110.1535467360958602No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC82500.15060760584839455Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACTC79850.14576990699387038Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT78930.14409040399531858Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAATGTGGCGCTCCGCGGTTGAGATCGGAAGAGCACACGTCTGAACTCC78930.14409040399531858Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGATTGGTGCACTGGAAGAAAGCTAGATCGGAAGAGCACACGTCTGAAC78920.144072148527943Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG78850.14394436025631405No Hit
AAATTGTGGCCACCGACTTTCTGGAAGAGATCGGAAGAGCACACGTCTGA77970.1423378791272645Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA76670.13996466836844135No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG76300.1392892160755455No Hit
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT70960.12954079649699488Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCTG66400.12121630337373816Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGTGGCCACCGACTTTCTGGAAGAGATCGGAAGAGCACACGTCTGAAC66130.12072340575459796Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA63330.11561187488944033No Hit
AAGACTGACTAAGCATGTAGTACCGAGGAGATCGGAAGAGCACACGTCTG62910.11484514525966667Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC62340.11380458361925959Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTCCGGATCGTCCGAGCTTTGATAAAGCATTTCAGATCGGAAGAGCACA61320.11194252594695217No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA60900.11117579631717853Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAAC60870.11112102991505185Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG60470.1103908112200293No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC58300.10642937479953216Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGACGTGGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT57450.10487766007260929TruSeq Adapter, Index 18 (100% over 35bp)
AATTGTGGCCACCGACTTTCTGGAAGAGATCGGAAGAGCACACGTCTGAA56720.1035450109541932Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)