FastQCFastQC Report
Fri 14 Jul 2023
SRR9691543_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691543_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5423378
Total Bases412.1 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA1499832.765490437878385Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA1002981.849363994174848No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC755541.393116983547892No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC742941.369884230824405No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG732021.3497491784640496No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG458900.8461516051435102Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC430880.7944863883727079Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA419540.7735769109215695No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA321600.5929883552280516No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC257850.47544168966278955No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG245090.4519139178570994No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC232980.429584661072859No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC227290.4190930449620145No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA215440.39724319418635395Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA211400.38979396236072794No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC189430.34928415463572704Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT184100.3394563314598392Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT141180.2603174626588816No Hit
TTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACTC131970.2433354267395708Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTCCGGATCGTCCGAGCTTTGATAAAGCAGATCGGAAGAGCACACGTCT131650.24274538857516478Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT129870.23946330128565627Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT121800.22458327632704192Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACTGACCGTTGTTCCGCTGAGCGAGATCGGAAGAGCACACGTCTGAAC117140.21599084555787926Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA108420.19991230557781514Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT108370.19982011211462672Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA107520.1982528232404232Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTGACCGTTGTTCCGCTGAGCGAGATCGGAAGAGCACACGTCTGAACT105880.19522887764784236Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA102960.18984477939763741No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC101450.1870605368093465Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTGTGGCCACCGACTTTCTGGAAGAGATCGGAAGAGCACACGTCTGAAC99970.18433161029896863Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT94520.17428252281142861Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC93800.17295493694151506No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA90030.1660035498171066Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCA88410.16301648160980112Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT87820.16192859874417753Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGATTGGTGCACTGGAAGAAAGCTAGATCGGAAGAGCACACGTCTGAAC86970.16036130986997402Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGTTCCGTGGAAACTGCGTTGTCTGGAGATCGGAAGAGCACACGTCTGA86940.16030599379206095Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA85650.15792740244179918TruSeq Adapter, Index 10 (100% over 35bp)
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT83580.15411059306579775Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAAC83320.15363118705721784Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTCCGGATCGTCCGAGCTTTGATAAAGCAAGATCGGAAGAGCACACGTC79180.14599756830521493No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA78350.14446715681628683No Hit
ACTGACCGTTGTTCCGCTGAGCGAAGCAGATCGGAAGAGCACACGTCTGA76520.1410928760635899Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGACTGACTAAGCATGTAGTACCGAGGAGATCGGAAGAGCACACGTCTG76350.14077941828874918Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC75470.13915681333663263Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAAGATCTCGTATG74960.13821644001211053TruSeq Adapter, Index 7 (97% over 35bp)
AATTGTGGCCACCGACTTTCTGGAAGAGATCGGAAGAGCACACGTCTGAA72610.13388334724225381Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTTCCGTGGAAACTGCGTTGTCTGGCAGATCGGAAGAGCACACGTCTG66260.12217477741732181Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC65660.12106845585906052Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT65660.12106845585906052No Hit
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT65070.11998057299343692Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGGCTGAGGCGTGATGACGAGAGATCGGAAGAGCACACGTCTGAACTCC63830.1176941751063636Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT62360.11498368728862345Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC57710.10640969521209843No Hit
ATGATTGGTGCACTGGAAGAAAGCAGATCGGAAGAGCACACGTCTGAACT55440.10222411198334322Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT54350.1002142944858352No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)