FastQCFastQC Report
Fri 14 Jul 2023
SRR9691548_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691548_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758905
Total Bases133.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC18267310.38560922846885Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT362462.060713910074734Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA287011.6317538468535822Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA186741.0616832631665725No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC186401.059750242338273No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA181041.0292767375156702Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC158380.9004465846648909No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG157620.8961257145781041No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG144980.8242628226083842No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG126220.7176055557292748No Hit
TTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCC112380.6389202373067335Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTC94070.5348213803474321Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA88210.5015051978361538No Hit
TCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCCA84150.4784226550041077Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA83670.4756936844229791No Hit
AGTTGTGAACTTCAGCACCCATGATGCAGGAGATCGGAAGAGCACACGTC80410.457159425892814No Hit
ATGCAGGTGGTCTGACCCTGAATGACTAGATCGGAAGAGCACACGTCTGA78120.4441399620786797Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCGTCGTCCGTAATAACAGTAAAGGAGATCGGAAGAGCACACGTCTGA67190.38199902780423045Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC66490.3780192790400846No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT62680.3563580750523763Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA62470.3551641504231326No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG61280.34839857752408454No Hit
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT50490.2870535930024646Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT48340.2748300789411594No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT47580.27050920885437246Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACTC38720.2201369602110404Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35990.20461594003087147No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC33270.1891517734044761No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC32740.18613853505447991No Hit
TTGTGAACTTCAGCACCCATGATGCAGGAGATCGGAAGAGCACACGTCTG32160.1828410289356162Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGAA31480.17897498727901734Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA31190.1773262342195855No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT30520.17351704611676014No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA29570.16811595850827646No Hit
ACCTATCGTTTCGCCAACTATCATGAAACCAGATCGGAAGAGCACACGTC28710.16322655288375437No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA28610.16265801734601926No Hit
TTTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT28320.16100926428658738Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC27220.154755373371501No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTCACATC25870.14708014361207683TruSeq Adapter, Index 27 (97% over 37bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT25760.1464547545205682Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC25620.14565880476773901No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA25190.14321410195547798No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC24850.14128108112717858Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC24830.14116737401963153No Hit
AGTTGTGAACTTCAGCACCCATGATGCAGGTGAGATCGGAAGAGCACACG23100.13133170921681386No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC22710.1291144206196469No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG22510.12797734954417664No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG22170.1260443287158772Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA22140.12587376805455666No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA22070.1254757931781421Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTT21870.12433872210267183TruSeq Adapter, Index 12 (97% over 36bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG20980.11927875581682923No Hit
AAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT20920.11893763449418815Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCTGAACT20740.11791427052626492Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA19800.11257003647155474No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT19630.11160352605740505No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT19560.11120555118099044Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTTTCGTACTGAAACATCTTAACCAGATCGGAAGAGCACACGTCTGAAC19080.10847658059986184Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG18260.10381458919043382No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA17720.10074449728666415Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTCACAT17690.10057393662534361TruSeq Adapter, Index 27 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)