FastQCFastQC Report
Fri 14 Jul 2023
SRR9691549_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691549_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794960
Total Bases136.4 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC18131410.101283594063377Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT361362.0131924945402684Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA285671.5915117885635335Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC193281.076792797611089No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA190181.0595222177652983No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA182621.0174042875607257Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC159710.8897691313455454No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG158560.883362303338236No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG147590.8222467353032937No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG123570.6884275972723626No Hit
TTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCC113640.6331060302179435Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTC93920.5232428577795606Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA89940.5010696617194812No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA85900.478562196372064No Hit
TCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCCA85020.4736595801577751Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGCAGGTGGTCTGACCCTGAATGACTAGATCGGAAGAGCACACGTCTGA82290.4584503275839016Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTGTGAACTTCAGCACCCATGATGCAGGAGATCGGAAGAGCACACGTC79830.44474528680304853No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC66230.3689775816731292No Hit
TTTCGTCGTCCGTAATAACAGTAAAGGAGATCGGAAGAGCACACGTCTGA66040.3679190622632259Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA64520.3594509069839996No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG62610.3488100013370772No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT61610.3432388465481125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT51260.2855773944823283Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT49300.27465793109595754No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT47660.26552123724205556Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACTC39920.22240049917546909Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC34260.19086776306992914No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC33360.18585372375986092No Hit
TTGTGAACTTCAGCACCCATGATGCAGGAGATCGGAAGAGCACACGTCTG32730.18234389624281322Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT31980.1781655301510897No Hit
TCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGAA31870.1775527031243036Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA30740.17125729821277352No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA29250.1629562775772162No Hit
ACCTATCGTTTCGCCAACTATCATGAAACCAGATCGGAAGAGCACACGTC28880.1608949503052993No Hit
TTTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT28710.1599478539911753Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA28590.15927931541649953No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA28230.15727369969247226No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTCACATC26740.14897267905691491TruSeq Adapter, Index 27 (97% over 37bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC26680.14863840976957704No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC26030.14501715915675Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT25890.14423719748629496Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC25540.14228729331015733No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC25350.14122877390025404No Hit
AGTTGTGAACTTCAGCACCCATGATGCAGGTGAGATCGGAAGAGCACACG23700.13203636849846237No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC23680.13192494540268307No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG23340.1300307527744351No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA23290.12975219503498686Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA22320.12434817488969113No Hit
AAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT22170.12351250167134643Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTGGGCACTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTT22110.12317823238400857TruSeq Adapter, Index 12 (97% over 36bp)
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG21820.12156259749520881Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG21300.1186655970049472No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT20880.11632571199358203No Hit
ATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCTGAACT20470.11404153853010653Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA20330.11326157685965148No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT19930.1110331149440656Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA19050.10613049872977671No Hit
ATTTTCGTACTGAAACATCTTAACCAGATCGGAAGAGCACACGTCTGAAC18760.10451486384097695Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG18540.10328920978740473No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)