FastQCFastQC Report
Fri 14 Jul 2023
SRR9691550_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691550_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773293
Total Bases134.7 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC17821610.050003016985913Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT352711.98901140420675Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA278121.5683815364973528Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC189401.0680694053379787No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA185551.046358385218912No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA178231.0050792508626605Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC158670.8947759902057922No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG154490.8712040255050914No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG145960.8231014276828477No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG123900.6987001020135984No Hit
TTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCC109300.6163674023412938Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTC90280.5091093237270997Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA88870.5011580150601169No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA84670.47747326583931704No Hit
TCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCCA83950.4734130231157513Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGCAGGTGGTCTGACCCTGAATGACTAGATCGGAAGAGCACACGTCTGA82080.4628676704864904Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTGTGAACTTCAGCACCCATGATGCAGGAGATCGGAAGAGCACACGTC80040.45136364943638757No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC65180.36756475100279534No Hit
TTTCGTCGTCCGTAATAACAGTAAAGGAGATCGGAAGAGCACACGTCTGA64410.36322254697898204Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA63350.3572449674137326No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG62340.35154934914873065No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT60510.3412295655596678Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT49960.28173573120742035Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT49210.27750631170370604No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT47750.2692730417364756Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACTC38940.2195914606328452Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC33040.18632002720362625No Hit
TTGTGAACTTCAGCACCCATGATGCAGGAGATCGGAAGAGCACACGTCTG32230.18175225413961482Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC31950.18017327085822818No Hit
TCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGAA31550.17791758045624723Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA31270.17633859717486056No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT31130.17554910553416722No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA31120.1754927132741177No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA29110.16415786900416343No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTCACATC28120.1585750352592606TruSeq Adapter, Index 27 (97% over 37bp)
ACCTATCGTTTCGCCAACTATCATGAAACCAGATCGGAAGAGCACACGTC27970.15772915135851773No Hit
TTTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT27620.15575542225678443Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA27500.15507871513619012No Hit
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC26320.14842442845034634No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT26310.14836803619029681Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC25700.14492810832727587No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC25500.14380026312628538Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC24220.13658205383994637No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC23930.13494667829851018No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA23340.13161953495558829Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTGTGAACTTCAGCACCCATGATGCAGGTGAGATCGGAAGAGCACACG23060.13004055167420162No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG22170.125021640529794No Hit
AAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT22070.12445771792929877Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG21660.12214563526726831Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA21630.12197645848711972No Hit
ATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCTGAACT21410.1207358287660302Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT21170.11938241452484163Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGCACTCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTT20940.1180853925437026TruSeq Adapter, Index 12 (97% over 36bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG20870.11769064672335593No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT20240.11413793434023593No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA20060.11312287365934451No Hit
ATTTTCGTACTGAAACATCTTAACCAGATCGGAAGAGCACACGTCTGAAC19230.10844231607523404Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA18820.10613023341320357No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG18340.10342340493082643No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA17990.1014496758290931Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTCACAT17850.10066018418839978TruSeq Adapter, Index 27 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)