FastQCFastQC Report
Fri 14 Jul 2023
SRR9691551_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691551_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022009
Total Bases153.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC21684010.723987875424887Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT389631.9269449344686398Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA356081.7610208460991026Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC277021.3700235755627201No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG212711.0519735569920807No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT172230.8517766241396552Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCC169960.8405501657015374Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA162350.8029143292636185Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA156550.774229986117767No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG153020.7567721014100334No Hit
TCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCCA144970.7169602113541532Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG137280.6789287288038778No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA134390.6646360129949965No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC133180.6586518655456034No Hit
TTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTC122810.6073662382313828Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA111630.5520746940295518No Hit
TTTCGTCGTCCGTAATAACAGTAAAGGAGATCGGAAGAGCACACGTCTGA109000.5390678280858295Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT92330.4566250694235288No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG71600.354103270559132No Hit
AGTTGTGAACTTCAGCACCCATGATGCAGGAGATCGGAAGAGCACACGTC70930.3507897343681457No Hit
ATGCAGGTGGTCTGACCCTGAATGACTAGATCGGAAGAGCACACGTCTGA70110.3467343617164909Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA61530.3043013161662485No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC54700.2705230293238062No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT49800.24628970494196614No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT48310.23892079609932498No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA45200.22354005348146325No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA41950.2074669301669775No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT40040.19802087923446432Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG39630.1959931929086369No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA38980.19277856824573977No Hit
ACCTATCGTTTCGCCAACTATCATGAAACCAGATCGGAAGAGCACACGTC38590.1908497934480015No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGAGATCGGAAGAGCACACGTC37210.18402489800985058No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC36810.18204666744806774No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA36290.17947496771775004Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC34890.17255116075151003No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC33600.16617136718976028No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC33570.16602299989762656No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA30760.152125930201102No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCACATC29150.14416355218992596TruSeq Adapter, Index 11 (97% over 42bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27760.13728920098773054No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA26690.13199743423496138No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC25840.1277936942911728Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC25690.1270518578305042No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA24060.11899056829123907No Hit
TTGTGAACTTCAGCACCCATGATGCAGGAGATCGGAAGAGCACACGTCTG23900.1181992760665259Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTC23570.11656723585305505No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT22980.11364934577442534Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT22780.11266023049353391No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG22700.11226458438117733No Hit
TCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGAA21580.10672553880818532Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG21360.10563751199920476No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG21320.10543968894302647No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG20550.10163159511159445Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT20290.10034574524643561Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)