FastQCFastQC Report
Fri 14 Jul 2023
SRR9691552_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691552_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2106025
Total Bases160 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC22158510.521480039410738Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT395371.8773281418786578Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA372271.767642834249356Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC290001.3770016975107133No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG229121.0879263066677747No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT181740.8629527189848173Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCC176500.8380717227953135Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA169050.8026970240144349Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA161130.7650906328272457No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG159070.7553091724932041No Hit
TCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTCCA147030.6981398606379317Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA141560.6721667596538502No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG141510.6719293455680725No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC139150.6607234007193646No Hit
TTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACTC124940.5932503175413397Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA115180.546907087997531No Hit
TTTCGTCGTCCGTAATAACAGTAAAGGAGATCGGAAGAGCACACGTCTGA111680.5302881019930913Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT95740.4546004914471576No Hit
ATGCAGGTGGTCTGACCCTGAATGACTAGATCGGAAGAGCACACGTCTGA78170.3711731817048705Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG73580.34937856863047684No Hit
AGTTGTGAACTTCAGCACCCATGATGCAGGAGATCGGAAGAGCACACGTC71360.33883738322194656No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA63760.30275044218373476No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC55090.26158283970988No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT51130.24277964411628544No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT50450.23955081254970856No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA47300.22459372514571288No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA45840.2176612338410038No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG42350.20108973065371968No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA41820.19857314134447598No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT40020.19002623425647844Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTATCGTTTCGCCAACTATCATGAAACCAGATCGGAAGAGCACACGTC39860.18926650918198976No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC39510.18760461058154582No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGAGATCGGAAGAGCACACGTC38180.18128939589985874No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA36900.17521159530394936Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC36470.17316983416626108No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC34550.16405313327239707No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC33570.15939981719115395No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCACATC31380.14900108023409028TruSeq Adapter, Index 11 (97% over 42bp)
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA30320.14396790161560286No Hit
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC27460.13038781590911788No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA26370.12521218883916382No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA26180.12431001531320854No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC25750.12226825417552024Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT24760.1175674552771216No Hit
AAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTC24420.11595303949383316No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT24260.1151933144193445Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG24180.11481345188210017No Hit
TTGTGAACTTCAGCACCCATGATGCAGGAGATCGGAAGAGCACACGTCTG24130.11457603779632246Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA22600.10731116677152455No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG22360.10617157915979154No Hit
TCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGAA21980.10436723210788096Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACTGCGACGACACATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT21560.1023729537873482Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)