FastQCFastQC Report
Fri 14 Jul 2023
SRR9691555_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691555_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4125414
Total Bases313.5 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC934512.2652514390070912No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA806331.954543228873514No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG805501.9525313095849288No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA600391.4553448453900628No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC422901.0251092375213737No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTAAATCTCGTATG325660.7893995608683153TruSeq Adapter, Index 6 (97% over 38bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG314270.7617902106309815No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC298080.7225456645078531No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA295910.7172855863678167Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA249880.6057089058213309No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG236750.5738817970753965No Hit
AGCGTTAGCGGTGATTGTCCGCTGCATCTGGATGAGATCGGAAGAGCACA217340.526831973712214No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT163770.39697833962845913Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC161230.39082138180555936No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC147970.3586791531710514Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTGACCCTGTGTCCGGAACTGAGCCAGATCGGAAGAGCACACGTCTGA136260.33029412320799806Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTGGTCCGTGTCAGGTTACCGTTGGCACCCTGGCAGATCGGAAGAGCA136110.3299305233365669No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT119310.2892073377362854Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG115660.28035974086479565No Hit
ATCGTCTGAGCCAGGCAGCACTGACCCTGCAGATCGGAAGAGCACACGTC106400.25791350880178326No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA88470.21445120417005423Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTCTGAGCCAGGCAGCACTGACCCTGCAAGATCGGAAGAGCACACGT84930.20587024720428057No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC80710.1956409708213527Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG78520.19033241269845888Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTGAGCCAGGCAGCACTGACCCTGCAAGATCGGAAGAGCACACGTCTGA77170.1870600138555791Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGTAGCCTGTGGGATCAGGATCGTCTGAGCCAGATCGGAAGAGCACAC74770.18124241591268173No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA73340.17777609713837206No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA72970.17687921745550872No Hit
TTAGCGGTGATTGTCCGCTGCATCTGGATGAAATTCGAGATCGGAAGAGC72860.17661257754979257No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA66880.16211706267540663No Hit
TGTCCGGAACTGAGCCTGGGTTGGTTTGAGATCGGAAGAGCACACGTCTG66360.1608565831211122Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG66070.16015362336967878No Hit
TCTGACCCTGTGTCCGGAACTGAGCCAGATCGGAAGAGCACACGTCTGAA63330.15351186571820427Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC62880.152421066103911No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC59160.14340378929242012No Hit
TCGTCTGAGCCAGGCAGCACTGACCCTGCAAGATCGGAAGAGCACACGTC58540.1419009098238383No Hit
TCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGAA56370.13664083168380192Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG56360.13661659169237317Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGAGCCAGGCAGCACTGACCCTGCAGATCGGAAGAGCACACGTCTGAA55290.1340229126094981Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA55250.13392595264378315No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC55190.1337805126952107Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGTAGCCTGTGGGATCAGGATCGTCTGAGATCGGAAGAGCACACGTCT54770.1327624330552037Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTCTGAGCCAGGCAGCACTGACCCTGCATAATCCAGATCGGAAGAGC54060.13104139366376322No Hit
ATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGAACTCC53800.13041115388661598Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA53730.1302414739466148No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACCAGATCGGAAGAGC51400.12459355594371861No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC48540.11766091839509926TruSeq Adapter, Index 6 (100% over 35bp)
TTGGTCCGTGTCAGGTTACCGTTGGCACCCTGGCAGATCGGAAGAGCACA47360.11480059940650805No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACCAGATCGGAAGAGCACA46420.11252204021220658No Hit
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT46240.11208572036648927Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGAGATCGG46140.1118433204522019No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT45740.11087372079505231No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACCAGATCGGAAGAG45140.10941932130932798No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG43370.10512884282644118No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA42220.10234124381213619Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGAAC41740.10117772422355671Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACTC41610.10086260433498309Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)