FastQCFastQC Report
Fri 14 Jul 2023
SRR9691556_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691556_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3919356
Total Bases297.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC884922.257819907148011No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA764681.951034812862113No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG757181.9318990160628429No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA576811.4716958602382635No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC397741.014809575858891No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTAAATCTCGTATG308690.7876038818622243TruSeq Adapter, Index 6 (97% over 38bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG297790.7597931905139518No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC285150.7275429943082486No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA282920.7218532840599323Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA231460.5905562036212071No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG223450.5701191726395868No Hit
AGCGTTAGCGGTGATTGTCCGCTGCATCTGGATGAGATCGGAAGAGCACA203400.5189628091962046No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT154950.39534556187291997Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC152130.38815050227639436No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC139550.3560533924450854Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTGACCCTGTGTCCGGAACTGAGCCAGATCGGAAGAGCACACGTCTGA127060.3241859121753676Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTGGTCCGTGTCAGGTTACCGTTGGCACCCTGGCAGATCGGAAGAGCA124500.3176542268678834No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT111850.28537851626644783Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG111020.283260821420662No Hit
ATCGTCTGAGCCAGGCAGCACTGACCCTGCAGATCGGAAGAGCACACGTC98610.2515974563168031No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA83690.21352997788412179Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTCTGAGCCAGGCAGCACTGACCCTGCAAGATCGGAAGAGCACACGT80740.20600323114307556No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC76580.19538924251841375Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG74640.19043944974633586Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTGAGCCAGGCAGCACTGACCCTGCAAGATCGGAAGAGCACACGTCTGA73580.1877349237987057Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA70560.18002957628753294No Hit
TTGGTAGCCTGTGGGATCAGGATCGTCTGAGCCAGATCGGAAGAGCACAC70360.17951928837288575No Hit
TTAGCGGTGATTGTCCGCTGCATCTGGATGAAATTCGAGATCGGAAGAGC70280.1793151732070269No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA70150.1789834860625062No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG63870.16296044554258401No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA63590.16224604246207797No Hit
TGTCCGGAACTGAGCCTGGGTTGGTTTGAGATCGGAAGAGCACACGTCTG62020.15824028233209742Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC60920.1554336988015378No Hit
TCTGACCCTGTGTCCGGAACTGAGCCAGATCGGAAGAGCACACGTCTGAA59760.15247402889658404Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTCTGAGCCAGGCAGCACTGACCCTGCAAGATCGGAAGAGCACACGTC56170.14331436082866675No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC55630.1419365834591193No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG54520.13910448553282734Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTAGCCTGTGGGATCAGGATCGTCTGAGATCGGAAGAGCACACGTCT54450.13892588476270082Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGAA54410.13882382717977138Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC53220.13578761408762052Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA53010.13525181177724094No Hit
ATCGTCTGAGCCAGGCAGCACTGACCCTGCATAATCCAGATCGGAAGAGC52030.13275140099546967No Hit
ATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGAACTCC50850.12974070229905116Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACCAGATCGGAAGAGC50260.12823535295084193No Hit
TCTGAGCCAGGCAGCACTGACCCTGCAGATCGGAAGAGCACACGTCTGAA50230.12815880976364483Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA49900.12731683470447697No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC46980.11986663115062782TruSeq Adapter, Index 6 (100% over 35bp)
TTGGTCCGTGTCAGGTTACCGTTGGCACCCTGGCAGATCGGAAGAGCACA44810.11433000727670566No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT44800.1143044928809733No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACCAGATCGGAAGAGCACA43740.11159996693334312No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACCAGATCGGAAGAG43060.10986498802354264No Hit
AAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGAGATCGG42970.10963535846195141No Hit
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT42510.10846169625826285Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG41750.10652260218260348No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA40430.10315470194593195Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT39480.10073083435135774No Hit
TTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACTC39360.10042466160256941Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)