FastQCFastQC Report
Fri 14 Jul 2023
SRR9691557_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691557_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4352755
Total Bases330.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC990432.2754094820406845No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA855281.964916472441017No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG843151.9370490643282243No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA632441.4529648464018765No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC435511.0005387392582399No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTAAATCTCGTATG340320.7818496561373199TruSeq Adapter, Index 6 (97% over 38bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG323750.7437818117491106No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC315030.723748522487482No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA302720.6954675831743344Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA266670.6126464733255146No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG240790.5531898762967362No Hit
AGCGTTAGCGGTGATTGTCCGCTGCATCTGGATGAGATCGGAAGAGCACA230180.5288145094313831No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC168120.3862381411313065No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT167210.3841475111739576Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC151320.34764189576486615Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTGACCCTGTGTCCGGAACTGAGCCAGATCGGAAGAGCACACGTCTGA142280.3268734399248292Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTGGTCCGTGTCAGGTTACCGTTGGCACCCTGGCAGATCGGAAGAGCA140930.32377195592216884No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT125870.2891731788258241Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG121710.2796160133065151No Hit
ATCGTCTGAGCCAGGCAGCACTGACCCTGCAGATCGGAAGAGCACACGTC110950.25489603710753306No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA94660.21747146347543106Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTCTGAGCCAGGCAGCACTGACCCTGCAAGATCGGAAGAGCACACGT87860.20184917368425281No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC84710.1946123776780453Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTGAGCCAGGCAGCACTGACCCTGCAAGATCGGAAGAGCACACGTCTGA81830.18799587847236982Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG80020.18383759251324736Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA77460.17795625988598027No Hit
TTGGTAGCCTGTGGGATCAGGATCGTCTGAGCCAGATCGGAAGAGCACAC76970.17683053606279242No Hit
TTAGCGGTGATTGTCCGCTGCATCTGGATGAAATTCGAGATCGGAAGAGC75880.17432637490508884No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA75590.17366013019340626No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA74760.1717532918806595No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG70060.1609555327602863No Hit
TGTCCGGAACTGAGCCTGGGTTGGTTTGAGATCGGAAGAGCACACGTCTG68090.15642966351195967Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTGACCCTGTGTCCGGAACTGAGCCAGATCGGAAGAGCACACGTCTGAA67700.15553367924452444Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC67620.15534988759992235No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC61230.14066952998732987No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG60150.13818834278520156Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGAA60050.13795860322944892Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTCTGAGCCAGGCAGCACTGACCCTGCAAGATCGGAAGAGCACACGTC59710.13717748873989002No Hit
ATCGTCTGAGCCAGGCAGCACTGACCCTGCATAATCCAGATCGGAAGAGC58990.13552336393847117No Hit
ATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGAACTCC58220.13375436935917595Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGTAGCCTGTGGGATCAGGATCGTCTGAGATCGGAAGAGCACACGTCT57880.13297325486961706Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGAGCCAGGCAGCACTGACCCTGCAGATCGGAAGAGCACACGTCTGAA57320.13168671335740237Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACCAGATCGGAAGAGC56840.1305839634897898No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA56800.13049206766748875No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC56210.12913660428854828Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA55920.1284703595768657No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC48720.11192911156267697TruSeq Adapter, Index 6 (100% over 35bp)
TTGGTCCGTGTCAGGTTACCGTTGGCACCCTGGCAGATCGGAAGAGCACA48420.1112398928954191No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACCAGATCGGAAGAGCACA48130.11057364818373651No Hit
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT48040.11036688258355914Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGAGATCGG47400.10889654942674237No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT47350.10878167964886605No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACCAGATCGGAAGAG47000.1079775912037319No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA45500.10453149786744256Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG44950.10326793031080317No Hit
TTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACTC43640.10025834213044382Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)