FastQCFastQC Report
Fri 14 Jul 2023
SRR9691559_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691559_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5954974
Total Bases452.5 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC1153791.9375231529138497No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTCAATCTCGTATG750071.2595688914846648TruSeq Adapter, Index 10 (97% over 36bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG561210.9424222507100786No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG503730.8458978998061117No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA461550.7750663562930754No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC445540.7481812682977289No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA438630.736577523260387Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA420640.706367483720332No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA320420.5380711989674514No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT289760.48658482807817466No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC265460.44577860457493185Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG241490.4055265396624738No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA229000.38455247663549835No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA220910.37096719481898666No Hit
AGCGTTAGCGGTGATTGTCCGCTGCATCTGGATGAGATCGGAAGAGCACA204330.34312492380319376No Hit
AGTTGGTCCGTGTCAGGTTACCGTTGGCACCCTGGCAGATCGGAAGAGCA198440.3332340325919139No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT175020.29390556533076384No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG174380.2928308335183327No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT172880.2903119308329474No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC160010.268699745792341No Hit
TTCTGACCCTGTGTCCGGAACTGAGCCAGATCGGAAGAGCACACGTCTGA158160.2655930991470324Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCATCATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTG154780.2599171717626307Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG141740.23801951108434732No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA141030.2368272304799316Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTCTGAGCCAGGCAGCACTGACCCTGCAGATCGGAAGAGCACACGTC137690.23121847383380684No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT135100.2268691685303748Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC132120.22186494852874253No Hit
ATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGAACTCC118070.1982712267089663Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTCCGGAACTGAGCCTGGGTTGGTTTGAGATCGGAAGAGCACACGTCTG115100.1932837993919033Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC112530.18896807945760974No Hit
ATCGTCTGAGCCAGGCAGCACTGACCCTGCAAGATCGGAAGAGCACACGT110960.1863316279802397No Hit
TCTGAGCCAGGCAGCACTGACCCTGCAAGATCGGAAGAGCACACGTCTGA107690.18084042012609963Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG99320.16678494314164932No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA94690.15900993018609316Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT93760.15744821052115424Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA93000.15617196649389234No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC92650.15558422253396909No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA89640.1505296244786291No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT88500.14861525843773626Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT85750.14399727018119643Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG83890.14087383085131858No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG83740.14062194058278005Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG78460.13175540313022358TruSeq Adapter, Index 4 (100% over 35bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCGGAAGAGCACA77530.13019368346528465No Hit
TTGGTCCGTGTCAGGTTACCGTTGGCACCCTGGCAGATCGGAAGAGCACA77320.1298410370893307No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG76960.12923650044483823No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA74680.12540776836305248No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC73570.12354378037586729Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA72190.12122638990531276No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA71790.12055468252254334No Hit
TCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAAC69890.11736407245438855Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTAGCGGTGATTGTCCGCTGCATCTGGATGAAATTCGAGATCGGAAGAGC68060.1142910111782184No Hit
TCTGAGCCAGGCAGCACTGACCCTGCAGATCGGAAGAGCACACGTCTGAA67490.11333382815777197Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTCTGAGCCAGGCAGCACTGACCCTGCAAGATCGGAAGAGCACACGTC66580.11180569386197152No Hit
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC66150.11108360842549438No Hit
TTGGTAGCCTGTGGGATCAGGATCGTCTGAGCCAGATCGGAAGAGCACAC66000.11083171815695586No Hit
TTGGTAGCCTGTGGGATCAGGATCGTCTGAGATCGGAAGAGCACACGTCT63800.10713732755172399Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA63020.1058274981553236Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA62830.10550843714850812Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGATCGTCTGAGCCAGGCAGCACTGACCCTGCATAATCCGCGAGATCGG62310.10463521755090786No Hit
TGTCCGGAACTGAGCCTGGGTTGGTTAGATCGGAAGAGCACACGTCTGAA61950.10403068090641537Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCATCATTCTTCTGGTGTAGATCTGGAGATCGGAAGAGCACACGTCTGA60860.10220027828836868Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGTTCTGGCAGTTCATCGTACCTTTCGTCAGATCGGAAGAGCACACGTC60480.10156215627473773No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)