FastQCFastQC Report
Fri 14 Jul 2023
SRR9691562_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691562_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6256241
Total Bases475.4 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC1212221.9376171729957334No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTCAATCTCGTATG772001.2339678091045405TruSeq Adapter, Index 10 (97% over 36bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG582280.9307186216132018No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG514210.8219152682896966No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA478390.7646604406703642No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC474180.7579311602606101No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA448310.7165804514244256No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA443310.7085884319354065Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA333840.5336111572428236No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT304710.48704965169979864No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC274360.438538093401453Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG248440.39710746437037825No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA244510.3908257370520094No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA233420.3730994378253651No Hit
AGCGTTAGCGGTGATTGTCCGCTGCATCTGGATGAGATCGGAAGAGCACA212760.34007641329673843No Hit
AGTTGGTCCGTGTCAGGTTACCGTTGGCACCCTGGCAGATCGGAAGAGCA205050.3277527192446711No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT182900.29234807290831666No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG181050.2893910256973796No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT178040.28457982996499015No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC167960.26846791867512776No Hit
TTCTGACCCTGTGTCCGGAACTGAGCCAGATCGGAAGAGCACACGTCTGA161700.2584619102748759Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCATCATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTG160730.2569114584940062Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG151130.24156678107508966No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA150120.2399523931383078Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTCTGAGCCAGGCAGCACTGACCCTGCAGATCGGAAGAGCACACGTC144030.2302181134006826No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC139720.22332899260114822No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT139660.22323308836727998Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGAACTCC123740.1977864983142433Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC118400.189251021499971No Hit
TGTCCGGAACTGAGCCTGGGTTGGTTTGAGATCGGAAGAGCACACGTCTG118020.18864362801880555Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTCTGAGCCAGGCAGCACTGACCCTGCAAGATCGGAAGAGCACACGT114000.18221804434963423No Hit
TCTGAGCCAGGCAGCACTGACCCTGCAAGATCGGAAGAGCACACGTCTGA112580.17994831081475282Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG102020.16306916565394458No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA101640.16246177217277916No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA98740.15782640086914812Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT97100.15520501847674986Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC95800.15312709340960493No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT94010.1502659504325361Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA93330.14917903578202948No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT87770.14029191011024034Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG86770.1386935062124365No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG85880.13727092674339111Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG81490.1302539336320324TruSeq Adapter, Index 4 (100% over 35bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCGGAAGAGCACA79730.1274407427718977No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA79420.1269452375635785No Hit
TTGGTCCGTGTCAGGTTACCGTTGGCACCCTGGCAGATCGGAAGAGCACA79340.1268173652517542No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC78450.12539478578270882Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG77920.1245476317168728No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA75590.12082335063498993No Hit
TCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAAC74320.11879337768477909Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA73590.11762654283938231No Hit
TTAGCGGTGATTGTCCGCTGCATCTGGATGAAATTCGAGATCGGAAGAGC72500.11588428259077614No Hit
TCTGAGCCAGGCAGCACTGACCCTGCAGATCGGAAGAGCACACGTCTGAA72270.11551664969428128Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTCTGAGCCAGGCAGCACTGACCCTGCAAGATCGGAAGAGCACACGTC68780.10993822009094599No Hit
TTGGTAGCCTGTGGGATCAGGATCGTCTGAGCCAGATCGGAAGAGCACAC68100.10885130544043939No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA67690.10819595984233983Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGTAGCCTGTGGGATCAGGATCGTCTGAGATCGGAAGAGCACACGTCT66450.10621393900906312Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC66240.1058782741905243No Hit
AGGATCGTCTGAGCCAGGCAGCACTGACCCTGCATAATCCGCGAGATCGG65500.10469545530614949No Hit
ATGTTCTGGCAGTTCATCGTACCTTTCGTCAGATCGGAAGAGCACACGTC65350.1044556947214789No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA63900.10213800906966339Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTCCGGAACTGAGCCTGGGTTGGTTAGATCGGAAGAGCACACGTCTGAA63480.10146667943258579Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCATCATTCTTCTGGTGTAGATCTGGAGATCGGAAGAGCACACGTCTGA62740.10028386054821098Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)