FastQCFastQC Report
Fri 14 Jul 2023
SRR9691563_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691563_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5842376
Total Bases444 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA770411.3186587100864444No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG577130.9878344016201628No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG568790.9735593874820793No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC553290.9470290854268879No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG288960.4945932956043911No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA255570.4374418900803372Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC253600.4340699742707419No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA246660.4221912454795788Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG202880.34725597941659353No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA200280.342805735200884No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA198970.3405634967691227No Hit
ATCCGAAAGTTACCTGCAAGCAAGAATGAGATCGGAAGAGCACACGTCTG174100.2979951992134707Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAGATCGGAAGAGCACACG172440.2951538894449793No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA170980.29265490615461925No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC160710.27507644150256677Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA156160.26728851412507515Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC150870.25823397877849696Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA145040.24825516194096373No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC144830.2478957191389257Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA142700.24424994214682522No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA136320.23332972749443037No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG133940.2292560424046655No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC126140.215905309757537No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG124300.2127559061587272No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC123000.21053078405087244Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC121980.20878491901240182No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC114850.19658098006701386Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA112930.19329464587695144No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT106510.18230596592893028Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG103200.17664046271585396No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC99730.17070109832027244No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG96250.16474461760078435No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG90410.15474868443934453No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA88700.1518217930513202No Hit
AGCTCTTGATCCGGCAAACAAACCACCGCTAGATCGGAAGAGCACACGTC88560.15158216451662815No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA87490.14975071785862465No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGCCATCTCGTATG83740.14333209639365901TruSeq Adapter, Index 20 (97% over 39bp)
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAAGAGATCGGAAGAGCA83290.14256186181786312No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT82790.1417060456225344No Hit
ATCCGAAAGTTACCTGCAAGCAAGAATAGATCGGAAGAGCACACGTCTGA81970.14030250706219524Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC80790.1382827808412194No Hit
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT79540.13614324035289754TruSeq Adapter, Index 18 (100% over 35bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG78630.1345856548773992No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA77360.13241188174126417No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA71550.1224672975515441Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT71490.12236459960810464Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA71100.12169706297574823No Hit
TTTTCTGAAAGAACGTACCGGTTTTACCAGATCGGAAGAGCACACGTCTG68520.11728105140785187Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA68170.11668198007112175Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC66150.11322448264199361No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA62680.10728511824641207No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA62160.10639506940327016Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAGATCGGAAGAGCACAC61010.10442669215401405No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)