FastQCFastQC Report
Fri 14 Jul 2023
SRR9691575_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691575_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2542557
Total Bases193.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG1327625.221593852173226No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG255991.0068210860169506No Hit
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAC217040.8536288468655766TruSeq Adapter, Index 7 (97% over 35bp)
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC200320.7878682759127916No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC183740.7226583317502813No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA141830.5578242690331032No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG116390.45776751514322No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC103870.4085257478986705Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG88590.34842876678870915No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA85440.3360396640075326No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAA84980.3342304616966306No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC83180.3271509743931011Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC71680.2819209166205517Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA65570.257889990273571Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA63450.24955192744941412No Hit
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGA63310.24900130065913958No Hit
AGGGAGTATGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT61230.2408205597750611Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG60210.23680885030306106No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA57070.2244590780069041TruSeq Adapter, Index 10 (100% over 35bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC54440.21411516044674714Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC51060.20082145651011954No Hit
TCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGT48530.19087084380015867Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA47250.1858365417176488No Hit
TTCAGACTCTGAACGCTAAAGTTGAGATCGGAAGAGCACACGTCTGAACT46870.1843419832869037Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC46430.18261144194604093No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAAGATCGG45390.17852107150400168No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAAGATCTCGTATG42700.16794117103372708TruSeq Adapter, Index 7 (97% over 35bp)
AATCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCA39690.156102695042825Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA39360.15480478903717793No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA36060.14182572898070722No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAGATCGGAAGA35710.14044916200502092No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA35170.1383253158139621Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTCTTGATCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA33740.1327010564561581TruSeq Adapter, Index 10 (100% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33320.13104917608533456No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT32570.12809938970886395Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAAGATC32020.12593621303278552TruSeq Adapter, Index 7 (97% over 35bp)
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA31760.12491362042227569No Hit
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGG31540.1240483497518443No Hit
TTCTTGGACTGGACCATAAAATTTCAGATCGGAAGAGCACACGTCTGAAC30800.1211378938603933Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGATCCACGTAGATGTAAACTAGATCGGAAGAGCACACGTCTGAACTC29160.11468769431717755Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAAGATCT28830.11338978831153049TruSeq Adapter, Index 7 (97% over 35bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA28130.1106366543601579Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATCTTCAGGAGATCGTCATGGACAGATTCCCACGAGATCGGAAGAGCA26910.1058383351877657No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT26580.10454042918211863Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAGATCGGAAG26250.10324252317647155No Hit
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG26240.10320319269145196Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTCATCTCCGGGGGTAGAGCAGATCGGAAGAGCACACGTCTGAACTCCA25580.10060738068015782Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGAGTATGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA25540.10045005874007938TruSeq Adapter, Index 10 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)