FastQCFastQC Report
Fri 14 Jul 2023
SRR9691577_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691577_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2677715
Total Bases203.5 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG1420785.30594181979785No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG279431.0435389875322802No Hit
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAC227570.8498663972827579TruSeq Adapter, Index 7 (97% over 35bp)
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC214730.8019150656436552No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC198350.7407435070573232No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA148890.5560337825347358No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG125460.4685338058755319No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC109370.40844526023120464Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG94870.3542946131309717No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAA93260.3482820240391528No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA92990.3472737016448726No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC87440.3265470746513352Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC75050.2802762803360328Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGA69860.2608940832015356No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA67930.25368644534612533No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA67230.25107227617576927Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG65830.24584393783505712No Hit
AGGGAGTATGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT65050.24293100647380322Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA59050.2205238421564655TruSeq Adapter, Index 10 (100% over 35bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC57320.2140631097782998Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC54510.20356908782301328No Hit
TCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGT51530.19244019621206887Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCAGACTCTGAACGCTAAAGTTGAGATCGGAAGAGCACACGTCTGAACT50330.18795876334860132Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA49720.18568070164300532No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC49190.18370140212830716No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAAGATCGG48290.1803403274807065No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAAGATCTCGTATG46840.1749252627706832TruSeq Adapter, Index 7 (97% over 35bp)
AATCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCA43800.16357229951656543Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA42160.15744767460315978No Hit
AGCTCTTGATCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA41870.15636466166115512TruSeq Adapter, Index 10 (100% over 35bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAGATCGGAAGA39720.14833542778077577No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA38500.14377930436958378No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA36500.13631024959713786Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACGATCCGGTACGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA36190.13515254610740873Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT34660.12943871920648764Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAAGATC34310.1281316346213096TruSeq Adapter, Index 7 (97% over 35bp)
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGG33690.12581622764185135No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA33390.12469586942598447No Hit
TTCTTGGACTGGACCATAAAATTTCAGATCGGAAGAGCACACGTCTGAAC33270.12424772613963771Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGATCCACGTAGATGTAAACTAGATCGGAAGAGCACACGTCTGAACTC31100.11614380171153389Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA30250.11296945343324438Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAAGATCT29250.10923492604702142TruSeq Adapter, Index 7 (97% over 35bp)
ATATCTTCAGGAGATCGTCATGGACAGATTCCCACGAGATCGGAAGAGCA28990.10826394892660346No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT28790.10751704344935888Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAGATCGGAAG28550.10662075687666536No Hit
AGGGAGTATGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA28330.10579916085169633TruSeq Adapter, Index 10 (100% over 35bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA27920.1042680046233449No Hit
ATTCATCTCCGGGGGTAGAGCAGATCGGAAGAGCACACGTCTGAACTCCA27880.10411862352789598Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG27540.10284888421658019Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)