FastQCFastQC Report
Fri 14 Jul 2023
SRR9691578_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691578_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2705723
Total Bases205.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG1429245.282284993696694No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG284791.0525467684607774No Hit
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAC233290.8622094722926182TruSeq Adapter, Index 7 (97% over 35bp)
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC219090.8097281207278055No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC201070.7431285464180923No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA152050.5619570074246328No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG126210.4664557310559876No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC113530.4195921016305069Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG95590.35328819690707436No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA94000.3474117638797467No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAA92850.3431615135769626No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC88620.327527984202374Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC74920.2768945675518152Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGA69540.2570107878744424No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA69150.25556939864132433Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA68290.2523909505888075No Hit
AGGGAGTATGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT67290.24869508076029956Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG66200.24466658264722588No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA58780.21724322851969696TruSeq Adapter, Index 10 (100% over 35bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC57240.21155158898379472Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC53670.19835733369602135No Hit
TCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGT52020.19225914847898326Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCAGACTCTGAACGCTAAAGTTGAGATCGGAAGAGCACACGTCTGAACT51390.18993075048702324Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA50790.18771322858991848No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAAGATCGG50740.1875284350984931No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC49590.18327818479570895No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAAGATCTCGTATG47190.1744080972072899TruSeq Adapter, Index 7 (97% over 35bp)
AATCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCA43000.1589224026258416Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA42960.15877456783270127No Hit
AGCTCTTGATCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA42380.15663096333216667TruSeq Adapter, Index 10 (100% over 35bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAGATCGGAAGA39030.14424979940666507No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA38140.14096047525929298No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA37960.14029521869016157Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA35900.13268172684343518No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAAGATC35640.13172080068802314TruSeq Adapter, Index 7 (97% over 35bp)
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGG35070.1296141548857736No Hit
AGACGATCCGGTACGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA33670.12443993712586247Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT33400.12344205227216533Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTTGGACTGGACCATAAAATTTCAGATCGGAAGAGCACACGTCTGAAC33130.1224441674184682Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGATCCACGTAGATGTAAACTAGATCGGAAGAGCACACGTCTGAACTC31330.1157916017271539Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAAGATCT30560.11294578195920277TruSeq Adapter, Index 7 (97% over 35bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA29670.10965645781183071Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGAGTATGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA29300.10828898597528276TruSeq Adapter, Index 10 (100% over 35bp)
ATATCTTCAGGAGATCGTCATGGACAGATTCCCACGAGATCGGAAGAGCA29050.10736501851815577No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT28870.10669976194902435Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAGATCGGAAG28700.106071464078178No Hit
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG28520.10540620750904657Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA27510.10167337898225354No Hit
ATTCATCTCCGGGGGTAGAGCAGATCGGAAGAGCACACGTCTGAACTCCA27250.10071245282684148Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)