FastQCFastQC Report
Fri 14 Jul 2023
SRR9691579_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691579_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12910245
Total Bases981.1 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA1735611.344366431465863No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC1070230.8289772967128045No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA1036870.8031373533190114No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA967600.7494822910022234No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG919700.7123799741987856No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG855350.6625358387853987No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC753930.5839780732278899Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG666430.5162024423239063No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC662790.5133829760783006No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT536630.41566213499433974Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA483740.3746946707827776Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA478270.37045772562797996Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA473550.3668017144523594Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG414210.32083821801987494No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTGATCTCGTATG412930.3198467573620795TruSeq Adapter, Index 9 (97% over 49bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA409340.31706602004841894Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG360380.2791426498877442No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA348350.26982446886174505No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA339270.26279129482050884No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG318180.2464554313260515No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA315900.24468939202935344No Hit
AGTTGTGTGGACCGCAAAACAGCATTTTAGCAGATCGGAAGAGCACACGT297820.23068501023799315No Hit
AGCGTTAATGGTCGTAGCGTTCTGACCGCATTTGATCTGAGATCGGAAGA295980.22925978554241225No Hit
AGCTCTTGATCCGGCAAACAAACCACCGCTAGATCGGAAGAGCACACGTC273130.21156066364348622No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA260250.20158409077441986No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC256840.19894277761576176No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC255190.19766472286157233No Hit
TTGTGTGGACCGCAAAACAGCATTTTAGCAGATCGGAAGAGCACACGTCT246180.1906857693250593Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA244580.18944644350281503No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT235810.1826533888396386No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGTTGAGATCGGAAGA230780.17875725828595818No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG219780.1702368932580288No Hit
ATCTGCTGGAACGTATTTATCATACCTGTAAACCGGGAGATCGGAAGAGC208390.16141444256092738No Hit
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG203810.15786687239475317No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC201810.1563177151169478Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA198040.15339755364828475No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG194540.1506865284121254No Hit
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA191230.14812267311735758TruSeq Adapter, Index 9 (100% over 35bp)
GGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCAC180120.13951710443914891No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT177710.13765036991939347No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA170160.13180230119567832Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA168260.13033060178176326Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA167650.1298581088120326No Hit
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC165620.1282857141750602No Hit
AGCGTTAATGGTCGTAGCGTTCTGACCGCATTTGATCAGATCGGAAGAGC160550.12435860047582366No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT160230.1241107353113748Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCACTTCACTCTGAAGTCTGACGTTCTGAGATCGGAAGAGCACACGTCT158540.1228016974116293Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG155260.12026107947602854Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAAGAGATCGGAAGAGCA153280.11872741377100125No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA149830.11605511746678704Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG146240.11327438015312646No Hit
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA144670.11205829169004926No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC144320.11178718916643332Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA141080.10927755437638868No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG140460.10879731562026902No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA136850.10600108673383038No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG136010.10535044067715214No Hit
ACTCGGCTGCGAACCGAGCGGTCGGAGGTTCGAGATCGGAAGAGCACACG133960.10376255446740168No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA132430.10257744914988058TruSeq Adapter, Index 9 (100% over 35bp)
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA131410.10178737893819986No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC130090.10076493513484833Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)